Prokaryotic Expression and Polyclonal Antibody Preparation of the Conserved Region of Yak Prdm9 Gene

Abstract

Abstract: The conserved region of Prdm9 cDNA was obtained from yak testis by RT-PCR and cloned into pMD19-T vector．Following sequence confirmation, the cDNA was ligated with pET-32a(+)vector and then transformed into E. coli BL21(DE3)to construct expression strains．The recombinant PRDM9 protein was efficiently expressed in the form of fusion protein through induction with IPTG．After purification by Ni-NTA, the recombinant protein was injected into Japan White rabbits, and polyclonal antibody against the conserved region of PRDM9 of yak was obtained and the antiserum was collected after the titter reach 1∶4, the antibody can be used in the future research．

Key words: Prdm9, Yak, Male infertility hybrid, Prokaryotic expression, Polyclonal antibody

Zeng Qin, Huang Lin, Lin Yaqiu, Jin Suyu, Zheng Yucai. Prokaryotic Expression and Polyclonal Antibody Preparation of the Conserved Region of Yak Prdm9 Gene[J]. Biotechnology Bulletin, 2014, 0(3): 155-158.

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