Studies on the Resistance of Transgenic Arabidopsis Overexpressing PcRS to Colletotrichum higginsianum

Abstract

Abstract: In order to reveal the effectiveness of Polygonum cuspidatum resveratrol synthase（PcRS）gene in increasing tolerance to pathogenic microorganisms in foreign species, the PcRS transgenic Arabidopsis was used as material to identify its molecular characterization and study its resistance to Colletotrichum higginsianum. Integration and expression of transgene in the genome of Arabidopsis was confirmed by Southern blot and Northern blot analyses. Independent homozygous transgenic Arabidopsis lines with genetical stability were obtained after the selection of T₃progenies and tested in vitro for disease resistance to C. higginsianum. Agar-plate bioassays were used to test the extracts of transgenic Arabidopsis for antifungal activity against C. higginsianum. Results showed that the mycelial growth of C. higginsianum was greatly inhibited. Then the detached leaves of 4-week-old Arabidopsis plants were used to inoculate. The lesion diameter and spore production significantly reduced on transgenic Arabidopsis. Overexpression of PcRS in transgenic Arabidopsis could restrict colonization of C. higginsianum by inhibition of spore production, resulting in enhanced resistance against C. higginsianum.

Key words: Polygonum cuspidatum, Resveratrol synthase, Transgenic Arabidopsis, Colletotrichum higginsianum, Disease resistance

Liu Zhongyu, Zhao Shujin. Studies on the Resistance of Transgenic Arabidopsis Overexpressing PcRS to Colletotrichum higginsianum[J]. Biotechnology Bulletin, 2014, 0(10): 107-112.

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