Toxicity of Beta-cypermethrin for Zebrafish Embryos

Abstract

Abstract: Beta-cypermethrin, an insecticide of type II pyrethroids, is highly toxic to fish and other aquatic organism and the residues can be detected in many foods. The impact of beta-cypermethrin for human health can not be neglected. To better define the toxicity mechanism of beta-cypermethrin for fish and human, the embryos of zebrafish were exposed to different sub-lethal concentrations of 0.05, 0.1, 0.2, 0.6 and 1 mg/L of beta-CYP solutions. With the help of a microscope, we observed the morphological changes. The results displayed that exposed to beta-CYP caused the embryonic toxicity increased in a dose-dependent manner. We chose zebrafish embryos exposure to beta-CYP（0.1 mol/L）as a model system to investigate the toxic effects on early development. A two-dimensional difference gel electrophoresis（2-D DIGE）coupled with MALDI-TOF-MS/MS analysis was employed to detect and identify the differential expressed proteins. In the present study, a total of 11 different proteins were successfully identified, which were mostly associated with cytoskeletal, stress response and metabolism. This study brings useful information and contributes to a better understanding about the embryo toxicity organisms of beta-CYP.

Key words: Zebrafish, Embryos, Beta-cypermethrin, DIGE, MALDI-TOF-MS/MS

Wang Jian, Liu Lili, Yu Kaimin, Li Guochao, Wu Wei, Yan Yanchun. Toxicity of Beta-cypermethrin for Zebrafish Embryos[J]. Biotechnology Bulletin, 2014, 0(10): 223-229.

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