Polymorphisms and Expression of Macrophage Migration Inhibitory Factor Gene from Channel Catfish,Ictalurus punctatus

Abstract

Abstract: Macrophage migration inhibitory factor（MIF）was considered as a unique cytokine produced by T-cell and involved in immune response to inflammation and stress. In this study, the genomic sequence of MIF was identified, and the polymorphisms and expression level were studied in channel catfish Ictalurus punctatus. The genomic sequence of MIF was identified to be 3 918 bp, consisting of a 70 bp 5'-untranslated region（UTR）, a 160 bp 3'-UTR, a 348 bp open reading frame（ORF）, two introns as 243 and 1 441 bp, and a 1382 bp promoter. Four repetitive sequences were found in promoter and introns. Sixty sites of single nucleotide polymorphisms（SNPs）were identified in MIF genomic sequence, among which 6 sites were found to locate in the binding sites for transcription factor. MIF was found to be most abundantly expressed in intestine, and weaker in other tissues. These results could give more information and greater understanding of the degree of MIF genetic variation.

Key words: Channel catfish, Ictalurus punctatus macrophage migration inhibitory factor, Genomic sequence, Promoter polymorphism, Tissue expression

Gao Lei, Du Xiaoxi, Li Le, Gao Xianggang, Bao Xiangbo, He Chongbo. Polymorphisms and Expression of Macrophage Migration Inhibitory Factor Gene from Channel Catfish,Ictalurus punctatus[J]. Biotechnology Bulletin, 2014, 0(10): 215-222.

References

[1] Bloom BR, Bennett B. Mechanism of a reaction in vitro associated with delayed-type hypersensitivity[J]. Science, 1966, 153（3731）：80-82.
[2] David JR. Delayed hypersensitivity in vitro its mediation by cell-free substances formed by lymphoid cell-antigen interaction[J]. Proc Natl Acad Sci USA, 1966, 56：72-77.
[3] Weiser W, Temple PA, Witek-Giannotti JS, et al. Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor[J]. PNAS, 1989, 86（19）：7522-7526.
[4] Calandra T, Roger T. Macrophage migration inhibitory factor：a regulator of innate immunity[J]. Nat Rev Immunol, 2003, 3（10）：791-800.
[5] Bernhagen J, Calandra T, Mitchell RA, et al. MIF is a pituitary-derived cytokine that potentiates lethal endotoxaemia[J]. Nature, 1993, 365（6448）：756-759.
[6] Lue H, Kleemann R, Calandra T, et al. Macrophage migration inhibitory factor（MIF）：mechanisms of action and role in disease[J]. Microbes Infect, 2002, 4（4）：449-460.
[7] Miska KB, Fetterer RH, Lillehoj HS, et al. Characterisation of macr-ophage migration inhibitory factor from Eimeria species infectious to chickens[J]. Mol Biochem Parasit, 2007, 151（2）：173-183.
[8] Suzuki M, Takamura Y, Maéno M, et al. Xenopus laevis macrophage migration inhibitory factor is essential for axis formation and neural development[J]. J Biol Chem, 2004, 279（20）：21406-21414.
[9] Ito K, Yoshiura Y, Ototake M, et al. Macrophage migration inhibitory factor（MIF）is essential for development of zebrafish, Danio rerio[J]. Dev Comp Immunol, 2008, 32（6）：664-672.
[10] Müller II, Müller KAL, Schönleber H, et al. Macrophage migration inhibitory factor is enhanced in acute coronary syndromes and is associated with the inflammatory response[J]. PLoS One, 2012, 7（6）：e38376.
[11] Nanji AA, Lau GK, Tipoe GL, et al. Macrophage migration inhibitory factor expression in male and female ethanol-fed rats[J]. Journal of interferon & cytokine research：the official journal of the International Society for Interferon and Cytokine Research, 2001, 21（12）：1055-1062.
[12] Shen Y, Thompson DL, Kuah MK, et al. The cytokine macrophage migration inhibitory factor（MIF）acts as a neurotrophin in the developing inner ear of the zebrafish, Danio rerio[J]. Dev Biol, 2012, 363（1）：84-94.
[13] Mao Y, Xu B, Su Y, et al. Cloning and mRNA expression of macrophage migration inhibitory factor（MIF）gene of large yellow croaker（Pseudosciaena crocea）[J]. Acta Oceanologica Sinica, 2010, 29（3）：63-73.
[14] Buonocore F, Randelli E, Facchiano AM, et al. Molecular and structural characterisation of a macrophage migration inhibitory factor from sea bass（Dicentrarchus labrax L.）[J]. Vet Immunol Immunop, 2010, 136（3-4）：297-304.
[15] Jin HJ, Xiang LX, Shao JZ. Molecular cloning and identification of macrophage migration inhibitory factor（MIF）in teleost fish[J]. Dev Comp Immunol, 2007, 31（11）：1131-1144.
[16] 金红建, 邵健忠, 项黎新. 红鳍东方鲀MIF基因的鉴定及生物信息学分析[J]. 水生生物学报, 2007, 31（1）：135-138.
[17] 王春平, 秦成路, 周芸, 等. MIF基因启动子区-794CATT微卫星多态性与子宫内膜异位症的相关性研究[J]. 中国妇幼保健, 2011, 26（7）：1086-1089.
[18] 李艳林, 邓少丽, 曾照芳. 人MIF基因-794CATT5-8微卫星多态性与结核病易感性关系的研究[J]. 激光杂志, 2011, 32（4）：62-63.
[19] 陈昌福. 关于我国斑点叉尾鮰养殖业发展的思考[J]. 渔业致富指南, 2007, 11：18-19.
[20] Livak KJ, Schmittgen TD. Analysis of relative gene expression data using Real-time quantitative PCR and the 2 -ΔΔCT method[J]. Methods, 2001, 25（4）：402-408.
[21] Li F, Huang S, Wang L, et al. A macrophage migration inhibitory factor like gene from scallop Chlamys farreri：Involvement in immune response and wound healing[J]. Dev Comp Immunol, 2011, 35（1）：62-71.
[22] Li W, Jin X, He L, et al. Molecular cloning, characterization and expression analysis of macrophage migration inhibitory protein（MIF）in Chinese mitten crab, Eriocheir sinensis[J]. Fish Shellfish Immunol, 2011, 30（1）：324-329.
[23] Nickel W. The mystery of nonclassical protein secretion[J]. Eur J Biochem, 2003, 270（10）：2109-2119.
[24] 柳爱华, 石梅, 赵勤, 等. MIF基因启动子多态性与结核病相关性研究进展[J]. 热带医学杂志, 2010, 10（9）：1143-1145.
[25] Donn RP, Shelley E, Ollier WER, et al. A novel 5'-flanking region polymorphism of macrophage migration inhibitory factor is associated with systemic-onset juvenile idiopathic arthritis[J]. Arthritis & Rheumatism, 2001, 44（8）：1782-1785.
[26] Barton A, Lamb R, Symmons D, et al. Macrophage migration inhibitory factor（MIF）gene polymorphism is associated with susceptibility to but not severity of inflammatory polyarthritis[J]. Genes Immun, 2003, 4（7）：487-491.
[27] Fingerle-Rowson G, Koch P, Bikoff R, et al. Regulation of macrop-hage migration inhibitory factor expression by glucocorticoids in vivo[J]. The American Journal of Pathology, 2003, 162（1）：47-56.
[28] Maaser C, Eckmann L, Paesold G, et al. Ubiquitous production of macrophage migration inhibitory factor by human gastric and intestinal epithelium[J]. Gastroenterology, 2002, 122（3）：667-680.
[29] Merk M, Mitchell RA, Endres S, et al. D-dopachrome tautomerase（D-DT or MIF-2）：Doubling the MIF cytokine family[J]. Cytokine, 2012, 59（1）：10-17.
[30] Oh M, Kasthuri SR, Wan Q, et al. Characterization of MIF family proteins：MIF and DDT from rock bream, Oplegnathus fasciatus[J]. Fish Shellfish Immunol, 2013, 35（2）：458-468.