Development of A Monoclonal Antibody Sandwich ELISA for Detection of bar Gene Transgenic Plant and Element

Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (10): 71-75.

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Development of A Monoclonal Antibody Sandwich ELISA for Detection of bar Gene Transgenic Plant and Element

Long Likun, Li Congcong, Zhang Ming, Li Feiwu

Jilin Academy of Agricultural Sciences,Changchun 130033

Received:2014-03-13 Online:2014-10-20 Published:2014-10-17

Abstract

Abstract: This study induced prokaryotic express Bar protein, by protein Immunity of BALB/c mice, cell fusion and screening clones, we obtained a hybridoma cell line. In this study, using rabbit polyclonal IgG and hybridoma cells secreting monoclonal antibodies we established sandwich ELISA method for detection of bar gene intransgenic plant which showed good specificity and sensitivity . This study provides rapid and accurate.technical tool for a secure monitoring of GMO.

Key words: bar gene, Hybridoma, Monoclonal antibody, GMO detection

Long Likun, Li Congcong, Zhang Ming, Li Feiwu. Development of A Monoclonal Antibody Sandwich ELISA for Detection of bar Gene Transgenic Plant and Element[J]. Biotechnology Bulletin, 2014, 0(10): 71-75.

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Development of A Monoclonal Antibody Sandwich ELISA for Detection of bar Gene Transgenic Plant and Element

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