Effects of Yeast Extract on Cell Growth and Antibody Production in CHO Cell Culture

doi:10.13560/j.cnki.biotech.bull.1985.2016-1020

Abstract

Abstract: The objective is to get insights into the effects of yeast extract on Chinese hamster ovary（CHO）cell growth and monoclonal antibody production. We comprehensively investigated the cell growth,antibody production and nutrients metabolism at different yeast extract concentrations in continuous passage culture and batch culture. It was found that yeast extract significantly improved CHO cell growth at low concentration（1 g/L）and remarkably inhibited CHO cell growth at high concentration（5-10 g/L）during continuous passage culture. Additionally,the result of seed cells in batch culture was not influenced while adding the yeast extract during continuous passage culture. Supplementing yeast extract in batch culture medium,a positive correlation was found between specific antibody production rate and the yeast extract concentration with a maximal antibody titer achieved at yeast extract concentrations of 10 g/L. In conclusion,yeast extracts can make a great contribution on animal cell culture process with a strategy of low concentration added at cell growth phase and high concentration added at protein-production phase.

Key words: animal cell culture, CHO cells, yeast extract, monoclonal antibody, amino acid metabolism

HU Dong-dong, ZHAO Liang, FAN Li, LIU Xu-ping, DENG Xian-cun, MIU Shi-wei, TAN Wen-song. Effects of Yeast Extract on Cell Growth and Antibody Production in CHO Cell Culture[J]. Biotechnology Bulletin, 2017, 33(6): 162-169.

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