Analysis on Genetic Diversity of Hainan Upland Rice Local Varietiesand Establishment of Molecular ID with SRAP Markers

Abstract

Abstract: SRAP markers were used to analyze the genetic relationships of 28 upland rice local varieties from Hainan province. Forty-six primer combinations with abundant polymorphism were selected from 255 primer combinations, and 18 primer combinations were picked to amplify the 28 material. A total of 180 bands were scored, and 160（87.75%）out of them were polymorphic, with an average of 10 bands and 8.9 polymorphic bands for each primer combination. Cluster analysis using UPMGA method showed that the genetic similarity coefficient ranged from 0.687 0 to 0.921 2 among the test upland rice varieties. The tested materials were classified into two clusters at the similarity coefficient of 0.687 0, and cluster I included Hainan shanlan upland rice；the cluster Ⅱ involved upland rice poya and varieties developed by our team. The clustering result of Hainan upland rice varieties showed close genetic relationship among Shanlan upland rice varieties. The Hainan upland rice poya might has some genetic similarities with nonglutinous rice germplasms. In addition, we established the molecular identity card of 28 Hainan upland rice varieties with SRAP markers. All these results provided the basis for the Shanlan upland rice heredity relationship and the Shanlan upland rice germplasm resources’ appraisal and the new variety’s breeding.

Key words: Upland rice , Shanlan upland rice , SRAP markers, Genetic diversity, Molecular identity card

Yao Yufei,Wang Ying,Zhuang Nansheng,Gao Heqiong. Analysis on Genetic Diversity of Hainan Upland Rice Local Varietiesand Establishment of Molecular ID with SRAP Markers[J]. Biotechnology Bulletin, 2014, 0(11): 97-106.

References

[1]叶凡, 杨小波, 党金玲, 等. 中国山栏稻的研究进展[J].作物杂志, 2008（2）：12-14.
[2] 王官远, 陈秦琼, 黄丹, 等. 海南稻种种质资源考察研究[C]//海南岛作物种质资源考察文集.北京：农业出版社, 1992：13-19.
[3]中国农业科学院. 中国稻作学[M].北京：农业出版社, 1986.
[4]郑成木, 黄东益, 陈辉, 等. 海南山栏稻种质类缘与杂交育种利用的研究[J].热带作物学报, 1998, 19（2）：74-81.
[5]郑成木, 黄东益, 莫饶. “热大99W”序列旱稻新品系农艺特征与抗旱特性的研究[J].热带作物学报, 2000, 21（4）：52-58.
[6]郑成木. 海南山栏稻遗传基础的研究-Ⅰ核仁动态与核仁组织者数目的变化[J].热带作物学报, 1995, 16（2）：110-114.
[7]郑成木, 陈辉, 饶世永. 海南山栏稻遗传基础的研究-Ⅱ核型与异染色质特征[J].热带作物学报, 1996, 17（1）：67-74.
[8]刘鸿艳, 王英, 郑成木. 335份旱稻核心样品的构建[J].热带作物学报, 2005, 26（1）：84-90.
[9]陈良斌, 朱汝财, 吴海滨, 等. 海南普通野生稻和山栏稻SSR多样性比较[C].植物分子国际研讨会论文集, 2005, 10（5）：27-30.
[10] 曾莉娟. 旱稻种质类缘鉴别与遗传基础RAPD分析[D].海口：华南热带农业大学, 2001：33-34.
[11]王英, 叶通, 邱海燕, 等. 49份旱稻种质RAPD标记遗传多样性分析[J].中国农学通报, 2011, 27（24）：21-28.
[12]Li G, Quiros CF. Sequence-related amplified polymorphism（SRAP）, a new marker system based on a simple PCR reaction：its application to mapping and gene tagging in Brassica[J].Theor Appl Genet, 2001, 103（2-3）：455-461.
[13]何凤发, 杨志平, 张正圣, 等. 马铃薯遗传资源多样性的SRAP分析[J].农业生物技术学报, 2007, 15（6）：1001-1005.
[14]李晓慧, 田朝阳, 王从彦. SRAP分子标记分析西瓜遗传多态性[J].生物技术, 2007, 17（3）：23-26.
[15]王燕, 龚义勤, 赵统敏, 等. 番茄SRAP-PCR体系优化与品种分子鉴定[J].南京农业大学学报, 2007, 30（1）：23-29.
[16] Esposito MA, Martin EA, Cravero VP. Characterization of pea accessions by SRAP’s markers[J]. Scie Hort, 2007（113）：320-335.
[17] Riaz A, Li G, Quresh Z, et a1. Genetic diversity of oilseed Brassica napus inbred lines based on sequence-related amplified polymorp-hism and its relation to hybrid performance[J]. Plant Breeding, 2001, 120（5）：411-415.
[18] 林忠旭, 张献龙, 聂以春, 等. 棉花SRAP 遗传连锁图构建[J].科学通报, 2003（48）：1676-1679.
[19]Ferriol M, Pico B, Nuez F. Genetic diversity of some accessions of cucurbitamaxima from spain using RAPD and SBAP markers[J].Genet Resour Crop Evo, 2003, 50（3）：227-238.
[20]Li G, Gao M, Yang B, et al. Gene for gene alignment between The Brassies and Arabidopsis genomes by direct transcriptase mapping[J]. Theor Appl Genet, 2003（107）：168-180.
[21]Chen W, Zhang Y, Liu XP. Detection of QTL for six yield-related　traits in oilseed rape（Brassica napus）using DH and immortalized F-2Populations[J]. Theor App Genet, 2007, 115（6）：849-858.
[22]Sun ZD, Wang ZN, Tu JX. An ultra-dense genetic recombination　map for Brassica napus, consisting of 13551 SRAP markers[J].Theor Appl Genet, 2007, 114（8）：1305-1317.
[23]Fu FY, Liu LZ, Chai YR. Localization of QTLs for seed color using recombine anti-nb redlines of Brassica napus in different environments[J]. Genome, 2007, 50（9）：849-854.
[24]Li G, Quris CF. Analysis, expression and molecular characterization of Bo GLS-ELONG, a major gene involved in the Aliphatic glucosi-nolate pathway of Brassica species[J]. Genetics, 2002（162）：1937-1943.
[25]Johansen B. In situ PCR on plant material with sub-cellular resolu-tion[J]. Ann Bot, 1997, 11（5）：697-700.
[26]徐鲜均. 黄麻属野生种与栽培种遗传资源多样性研究[D]. 福州：福建农林大学, 2009.
[27] 王呈丹, 牛俊海, 张志群, 等. 红掌品种亲缘关系SRAP分析[J]. 植物遗传资源学报, 2013, 14（4）：759-763.
[28]崔竣杰, 宋建文, 汪国平, 等. 丝瓜种质资源亲缘关系的SRAP分析[J]. 植物遗传资源学报, 2012, 13（6）：1061-1066.
[29]云天海, 郑道君, 谢良商, 等. 中国南瓜海南农家品种间的遗传特异性分析和DNA指纹图谱构建[J]. 植物遗传资源学报, 2013, 14（4）：679-685.
[30]Vandemark GJ, Ariss JJ, Bauchan GA, et al. Estimating genetic rel-ationships among historical sources of alfalfa germplasm and sele-cted cultivars with sequence related amplified polymorphisms[J]. Euphytica, 2006（152）：9-16.
[31] Guo DL, Luo ZR. Genetic relationships of some PCNA persimmons（Diospyros kaki Thunb. ）from China and Japan revealed by SRAP analysis[J]. Genet Resour Crop Evol, 2006（53）：1597-1603.
[32]Zhuang GQ, Ma DW, Wang WG, et al. Genetic diversity of Pogona-therum paniceum（Lam. ）Hack In Southwest China revealed by AFLP markers[J]. African J Biotechnol, 2009, 8（7）：1226-1232.
[33]吴渝生, 杨文鹏, 郑用琏. 3个玉米杂交种和亲本SSR指纹图谱的构建[J]. 作物学报, 2003, 29（4）：496-500.
[34]郑海燕, 粟建光, 戴志刚, 等. 利用ISSR和RAPD标记构建红麻种植资源分子身份证[J]. 中国农业科学, 2010, 43（17）：3499-3510.
[35]王晓飞, 陈建华, 栾明宝, 等. 苎麻种质资源分子身份证构建的初步研究[J]. 植物遗传资源学报, 2010, 11（6）：802-805.
[36]任羽, 王得元, 张银东. 相关序列扩增多态性（SRAP）一种新的分子标记技术[J]. 中国农学通报, 2004（6）：11-13.
[37]郑成木, 陈辉, 黄东益, 等. 海南山栏稻农艺特征与抗旱生理特性研究[J]. 热带作物学报, 1997, 18（2）：85-91.
[38]郑成木, 陈辉, 黄东益, 等. 海南山栏稻种质类缘与杂交育种利用的研究[J]. 热带作物学报, 1998, 19（2）：74-81.
[39]潘兆娥, 王希文, 孙君灵, 等. 中棉所48的SSR数字指纹图谱的构建[J]. 中国农学通报, 2010, 26（7）：31-35.
[40]郝冬梅, 邱财生, 于文静, 等. 亚麻RAPD标记分子身份证体系的构建与遗传多样性分析[J]. 中国农学通报, 2011, 27（5）：168-174.