Enhanced Oxidative Stress Tolerance of Transgenic Rice Plants Overexpressing OsPHGPx Gene

Abstract

Abstract: Plants can express phospholipid hydroperoxide glutathione peroxidase（PHGPx）to resist oxidative stress caused by biotic or abiotic stress. But the physiological role of PHGPx in conferring stress defence is still unclear. In this study, agrobacterium genetic transformation technology was successfully used to obtain transgenic rice overexpressing OsPHGPx. PCR, Real-time PCR and Western blot analysis was carried out on the transgenic rice, and all the results showed that OsPHGPx gene was transformed into the rice successfully. In addition, compared with the non-transgenic rice, seedlings of transgenic lines demonstrated enhanced tolerance to oxidative stress caused by paraquat.

Key words: OsPHGPx , ROS , Genetic transformation , Transgenic rice

Song Jianhui,Li Tian,Wu Xiuyun,Wu Fuqing,Liu Jinyuan. Enhanced Oxidative Stress Tolerance of Transgenic Rice Plants Overexpressing OsPHGPx Gene[J]. Biotechnology Bulletin, 2014, 0(11): 107-113.

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