Polymorphisms of TLR9 Gene in Sheep in Gansu Province

Abstract

Abstract: There is a acute and chronic respiratory disease in population of sheep，which leads to productive reduces even death. Gene TLR9 is associated with respiratory disease. The Polymorphism and variation of gene TLR9 was investigated in order to find the best alleles for respiratory disease resistance in the further observation by means of phenotype and genotype，reveal the genetic characteristics of sheep and provide data for future search. Fragments of DNA containing TLR9 were amplified using PCR. Single-strand conformational polymorphism(SSCP)analysis and DNA sequence analysis were employed to detect genetic variation in 427 healthy sheep and 58 with respiratory disease. Results showed that four alleles were identified，seven nucleotide polymorphisms were in the analysis of gene sequences，which mainly came from point mutation，including transition of 4(57.14%)and transversion of 3(42.86%). Sheep TLR9 gene in Gansu has high level of sequence polymorphism. TLR9 gene polymorphisms with respiratory disease in sheep has some relevance.

Key words: Sheep in, Gansu TLR9, Gene, Genetic, Polymorphism, PCR-SSCP

Lü Weili, Zhang Xiaoli, Ma Xiaojun, Zhang Guohua. Polymorphisms of TLR9 Gene in Sheep in Gansu Province[J]. Biotechnology Bulletin, 2014, 0(5): 107-111.

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