Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (5): 112-116.

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Comparison of Metallothionein-3 Gene Expression Levels in Different Brain Parts of Tibetan-sheep Between Different Altitudes Areas

Wang Jiali Wang Xinrong Zhao Shengguo Wang Jianfu Wu Jianping   

  1. (College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070)
  • Received:2013-10-20 Online:2014-05-23 Published:2014-05-24

Abstract: The study was set out to find the differences of Metallothionein-3(Metallothionein-3, MT-III)gene of Tibetan sheep in different altitudes areas and its expression differences in different parts of the brain tissue. Randomly selected 6 Tibetan rams from high altitude areas(Henan county, Qinghai)and 3 Tibetan rams form low altitude areas(Lintao county, Gansu), sampled various parts of the temporal lobe, parietal lobe, medulla, cerebellum and pineal gland in the brain tissue after slaughter, respectively, and analyzed the relative expression levels of MT-III gene at the brain tissue and other different parts of Tibetan sheep in different altitude areas by real-time PCR method. MT-III gene were expressed in various parts of brain tissue of Tibetan sheep, but there are differences of the relative expression levels in different parts;the relative expression of MT-III gene in pineal gland was significantly higher than that of other parts(P <0.01);at high altitude, the relative expression of MT-III gene in the temporal lobe, parietal lobe and medulla were significantly higher than those of Tibetan sheep at low altitude areas(P <0.01). MT-III is a protein that expressed in different parts of brain tissue of Tibetan sheep, its high expression in the temporal lobe, parietal lobe and medulla will cause the brain cells in this region enhanced tolerance to hypoxia and high radiation.

Key words: MT-III, Gene expression, Tibetan-sheep Different, Parts of the brain tissue, Different altitudes areas