Biocontrol Efficacy and Phylogenetic Tree Analysis of a New Bionectria ochroleuca Strain

Abstract

Abstract: The fruit and vegetable disease controls are more difficult because the increase of protected cultivation area. Biological control means became more and more popular in disease control process. By dual culture on PDA plates, WY-1 inhibited the growth of Botrytis cinerea, Cladosporium fulvum, Phytophthora parasitica, Vertillium dahliae, Fusarium wilt, Valsa sordida Nit., Alternaria and Pseudopeziza ribis Kleb. Scanning electron microscopy revealed that WY-1 inhibited the Botrytis cinerea growth significantly. 5.8S rDNA-ITS sequence alignment and neighbor-joining phylogenetic tree reveal that WY-1 belongs to Bionectria ochroleuca, which may provide a new choice in disease control.

Key words: Bionectria ochroleuca 5.8S, RDNA-ITS, Phylogenetic tree, Botrytis Cinerea

Chen Xiuling, Li Jingfu, Zhang Lili, Zhang Junfeng, Wang Aoxue. Biocontrol Efficacy and Phylogenetic Tree Analysis of a New Bionectria ochroleuca Strain[J]. Biotechnology Bulletin, 2014, 0(5): 184-189.

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