Isolation of Antifungal Substances from Bacillus amyloliquefaciens BA-26 and Its Antifungal Activity against Botrytis cinerea

doi:10.13560/j.cnki.biotech.bull.1985.2019-0055

Abstract

Abstract: The objective of this study are to analyze the antifungal substances produced by Bacillus amyloliquefaciens BA-26,to clarify the antifungal effects of these active substances,and to explore the mechanisms of their inhibition to plant pathogenic fungi. Antifungal substances produced by B. amyloliquefaciens BA-26 were isolated and purified by ammonium sulfate deposition,macroporous resin and RP-HPLC methods. Using the plate diffusion method,the inhibitory spectrum and the antifungal activities of these components were detected. The minimum inhibitory concentration of each purified component was determined by double dilution method,and their antifungal mechanisms to hyphae and spore germination were studied. The crude extract from strain BA-26 fermentation presented a broad inhibitory spectrum,and had significant inhibitory effect on various plant pathogenic fungi,such as Rhizoctonia solani and so on. Eleven antifungal components were isolated from the antifungal crude extract. The antifungal activity of A6-19 and A6-20 components showed the strongest antifungal activity against Botrytis cinerea,and the minimum inhibitory concentration was 7.81 μg/mL. These components inhibited the growth of B. cinerea mycelium,and resulted in them enlarged and thickened;in addition,they destroyed cell membrane,and inhibited spore germination. In this study,antifungal substances of B. amyloliquefaciens BA-26 were isolated and their mechanisms were preliminarily explored. These results laid the foundation for the application of these antimicrobial substances,especially in the field of anti-pathogenic fungi.

Key words: Bacillus amyloliquefaciens, antibacterialcompounds, Botrytis cinerea, bacteriostatic effects

LIU Chao, LIU Hong-wei, WANG Bu-qing, ZHAO Wen-ya, WANG Ya-na, ZHANG Li-ping. Isolation of Antifungal Substances from Bacillus amyloliquefaciens BA-26 and Its Antifungal Activity against Botrytis cinerea[J]. Biotechnology Bulletin, 2019, 35(7): 83-89.

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