The Improvement of Chicken Alpha Interferon Expression by a Recombinant Pichia pastoris with Cultural Condition Optimization

Abstract

Abstract: To achieve excellent performance of Pichia pastoris for chicken alpha interferon expression, pH value, temperature, culture volume and carbon source feeding pattern were optimized. The maximum anti-virus activity（3×10⁶ IU/mL）was harvested in the conditions of pH5.5, 28℃, and 40% culture volume in 10 L fermentor, as well as the glycerol-methanol（20∶1, V/V）co-feeding pattern was conducted when carbon source exhausted. This process was successfully scaled up to 50 L fermentor and the fermentation performance was also steady, which could be taken advantage of for its industrial application.

Key words: Pichia pastoris, Chicken alpha interferon, Optimization, Up-scale

Jiang Zhengjun,Liu Shuhai,Wang Maochao,Cheng Quanming,Huang Jin. The Improvement of Chicken Alpha Interferon Expression by a Recombinant Pichia pastoris with Cultural Condition Optimization[J]. Biotechnology Bulletin, 2014, 0(6): 115-119.

References

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