FST-related Genes Expression in FST Gene Knock-down Pig Fetal Fibroblast Cells

Abstract

Abstract: In order to obtain FST gene knock-down pig fetal fibroblast cells and investigate the effects of related gene’s expression, the short hairpin RNA eukaryotic expression vectors for FST was constructed and transfected into pig fetal fibroblast cells. After screened with G418, transfected stable cell clones were obtained, and Real-time PCR was used to detect FST-related genes change in gene’s expression. The results showed that in stably transfected cell lines, FST gene expression was significantly inhibited, then resulting in the expression of ActivinRⅡA, Myostatin and Bmp4 downward trend, and the expression of MyoD gene very significantly reduced.

Key words: Follistatin（FST）, RNA interference, Porcine fetal fibroblast cells, Related gene

Sun Yameng,Zhang Dongjie,Wang Liang,Zhang Xu,Yin Xue,Liu Di. FST-related Genes Expression in FST Gene Knock-down Pig Fetal Fibroblast Cells[J]. Biotechnology Bulletin, 2014, 0(6): 111-114.

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