Expression Analysis of Immune Genes at Different Induction Conditions of Third Instar Larvae of Musca domestica

Abstract

Abstract: We studied on the innate immune gene expression at different induction conditions of third instar larvae of Musca domestica. We chose the cold, heat stimulation and gram negative bacteria, gram positive bacteria and fungi induced the third instar larvae, 12 hours after the total RNA was extracted from third instar larvae.We designed the primers according to the published GenBank glyceraldehyde phosphate dehydrogenase（GAPDH）, Attacin, Cecropin, Defensin, Diptericin, Lysozyme, heat shock protein（HSP）and Musca antifungal peptide（MAF-1）gene sequences of housefly. We were using the GAPDH as reference gene. Analysed the gene expression in different inducing conditions of third instar larvae of housefly through RT-PCR reaction. Results showed that in different inducing conditions, the genes related innate immunity expression showed large differences. Microbial stimulation showed the higher levels of gene expression than physical stimulation. The gene expression induction of fungi and positive bacteria was the highest, the cold stimulation induced the lowest. The innate immune system of the housefly larvae could activate by both microbial and physical stimulation. In different conditions of stimulation, the body’s immune response to different stress.

Key words: Musca domestica, InductionInnate immunity, Gene expression

Xiu Jiangfan, Wei Chuanchuan, Chen Mingming, Wu Jianwei. Expression Analysis of Immune Genes at Different Induction Conditions of Third Instar Larvae of Musca domestica[J]. Biotechnology Bulletin, 2014, 0(6): 120-127.

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