Construction of a Mangrove Soil Metagenome Library and Identification of Two Novel β-Glucosidase Genes

Abstract

Abstract: Metagenomics provides a means of exploring new enzymes in microorganisms. A mangrove soil metagenome library with 100 000 clones was constructed. The average length of inserted DNA fragment is about 30 kb, and the total capacity of inserted DNA reached about 3 Gb. Seventeen clones with β-glucosidase activity were detected by screening 10 000 fosmid clones. The subcloning and sequencing revealed two novel β-glucosidase genes（MhGH3 and bgl66）. Bioinformatics analysis indicated that MhGH3 and bgl66 composed of 1 992 bp and 2 025 bp in length, respectively. The deduced amino acid sequence of MhGH3 and bgl66 showed the highest sequence identity of 64% and 55% with the known β-glucosidase in GenBank datebase, respectively.

Key words: β-Glucosidase, Cellulase, Mangrove, Metagenomic library

Mai Zhimao, Su Hongfei, Li Lizhen, Zhang Si. Construction of a Mangrove Soil Metagenome Library and Identification of Two Novel β-Glucosidase Genes[J]. Biotechnology Bulletin, 2014, 0(6): 168-172.

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