Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (8): 89-93.

Previous Articles     Next Articles

Relative Quantification of mRNA Transcription of TLR3 and TLR5 in Different Tissues of Yak

Chen Yabing1, Lan Daoliang2, Lin Baoshan1, Huang Cai1, Huang Yong1, Li Jian1,2   

  1. 1. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041;
    2. College of Tibetan Plateau Research, Southwest University for Nationalities, Chengdu 610041
  • Revised:2014-03-15 Online:2014-08-15 Published:2014-08-01
  • Contact: 通讯作者 : 李键,男,博士,教授,研究方向:动物生殖调控;E-mail:lijian@swun.cn;兰道亮,男,博士,副教授,研究方向:动物免疫机理;E-mail:landaoliang@163.com
  • Supported by:

    中央高校青年教师基金项目(2014NZYQN37)

Abstract:

According to the sequences of yak TLR3 and TLR5, the real-time PCR primers were designed to establish a method for the detection of mRNA transcription of TLRs by Real-time quantitative PCR, and the transcription level of TLRs in different tissues of yak was analyzed. The results showed that two genes have different expression pattern and expression level in tissues. The TLR3 mRNA was detected in examined tissues except mammary gland, and it was expressed at a higher level in heart, large intestine, stomach and muscle. The TLR5 mRNA level was higher in heart, lung, large intestine, small intestine, stomach, muscle and ovary. The results indicated that there exists difference in the transcription level of TLRs in tissues, which may be associated with the recognition of pathogens. For the same gene, the mechanism of gene may bring difference in expression level in different tissues.

Key words: Yak, Transcriptional level, TLRs, Relative quantification