Cloning and Sequence Analysis of Yak PAFR Gene and Expression in Uterus Before and After Pregnancy

Abstract

Abstract:

Platelet-activating factor receptor（PAFR）gene were cloned from uterus of domestic yak by reverse transcription polymerase chain reaction（RT-PCR）. The results shows that domestic yak PAFR gene was 1 029 bp in length, encoded predicted mature protein of 342 amino acids, which encoded a hydrophilic protein. Compared with the reference sequence of PCR primer designing homology of the domestic yak PAFR coding sequence was 99.7% at the amino acid level, and also showed high homology of with other species. The PAFR has 5 transmembrane domains, features G protein-coupled receptor domains, no signal peptide. The result of the quantitative Real-time Polymerase chain reaction（QRT-PCR）shows that PAFR was highest expression in gestation and lowest in follicular phase in uterus of domestic yak. That suggested PAFR play an important role in implantation and gestation.

Key words: Yak, PAFR, Gene cloning, Bioinformatic analysis, Gene expression

Wei Bo, Pan Yangyang, Yu Yao, Tian Feng, Yu Sijiu. Cloning and Sequence Analysis of Yak PAFR Gene and Expression in Uterus Before and After Pregnancy[J]. Biotechnology Bulletin, 2014, 0(8): 82-88.

References

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