Screening, Identification and Characterization of Strains Coupling Nitrogen Removal with Sulfide Removal

doi:10.13560/j.cnki.biotech.bull.1985.2015.10.029

Abstract

Abstract: In order to promote the engineering application of denitrification and desulfurization process, 2 highly-active autotrophic denitrifying bacterial strains H3 and H7 were screened from stable UASB with denitrification and desulfurization, and the strains were identified and characterized. Two strains were both gram negative bacteria, the 16S rDNA sequence analysis revealed that strain H3 and H7 had a similarity of 99.6%, 98.9% with Pseudomonas stutzeri CONC12 and P. stutzeri 19smn4 respectively. Combing the morphologic, physiobiochemical characteristics and the analysis of its 16S rDNA, 2 strains were identified as P. stutzeri. The results of growth and denitrification of 2 strains demonstrated that the optimal initial pH for H3 and H7 growth were 6.94 and 6.88 respectively, for denitrification were 6.77 and 6.56 respectively. The optimal temperatures for growth were 30.4℃ and 30.6℃, for denitrification were 31.4℃ and 31.2℃ respectively, both of H3 and H7 were non-resistant strains to salt.

Key words: desulfurization denitrifying bacteria, 16S rDNA, denitrification characteristic, non-resistant strain to salt

Huang Jun, Zhang Shiying, Wang Fanfan, Li Yuehan, Wu Peng, Song Yinling. Screening, Identification and Characterization of Strains Coupling Nitrogen Removal with Sulfide Removal[J]. Biotechnology Bulletin, 2015, 31(10): 184-190.

References

[1] Chen Y, Cheng JJ, Creamer KS. Inhibition of anaerobic digestion process：a review[J]. Bioresource Technology, 2008, 99（10）：4044-4064.
[2]Moore PK, Bhatia M, Moochhala S. Hydrogen sulfide：from the smell of the past to the mediator of the future?[J]. Trends in Pharmacological Sciences, 2003, 24（12）：609-611.
[3]Mahmood Q, Zheng P, Cai J, et al. Sources of sulfide in waste streams and current biotechnologies for its removal[J]. Journal of Zhejiang University Science A, 2007, 8（7）：1126-1140.
[4]Nica D, Davis JL, Kirby L, et al. Isolation and characterization of microorganisms involved in the biodeterioration of concrete in sewers[J]. International Biodeterioration & Biodegradation, 2000, 46（1）：61-68.
[5]Sublette KL, Sylvester ND. Oxidation of hydrogen sulfide by continuous cultures of Thiobacillus denitrificans[J]. Biotechnology and Bioengineering, 1987, 29（6）：753-758.
[6]Koenig A, Liu LH. Autotrophic denitrification of landfill leachate using elemental sulphur[J]. Water Science and Technology, 1996, 34（5）：469-476.
[7] 刘玲花, 王占生. 硫/石灰石滤柱去除地下水中硝酸盐的研究[J]. 环境工程, 1995, 13（3）：11-16.
[8]车轩, 罗国芝, 谭洪新, 等. 脱氮硫杆菌的分离鉴定和反硝化特性研究[J]. 环境科学, 2008, 29（10）：2931-2937.
[9]刘宏芳, 汪梅芳, 许立铭. 脱氮硫杆菌生长特性及其对 SRB 生长的影响[J]. 微生物学通报, 2003, 30（3）：46-49.
[10]范立民, 裘丽萍, 陈家长, 等. 养殖池塘系统脱氮硫杆菌（Thiobacillus denitrificans）的分离, 生长特性及脱氮特征研究[J]. 农业环境科学学报, 2013（1）：153-159.
[11]布坎南RE, 吉本斯NE. 中国科学院微生物研究所《伯杰细菌鉴定手册》翻译组译. 伯杰细菌鉴定手册[M]. 第8版. 北京：科学出版社, 1984：643-650.
[12]周国勤, 陈兵, 吴伟. 假单胞菌Pseudomonas stutzeri的生长特性及其反硝化活性[J]. 湛江海洋大学学报, 2004, 24（1）：46-50.
[13] 周晓黎, 孔瑛, 赵金生, 等. 石油生物脱硫菌Pseudomonas stutzeri UP-1的筛选[J]. 微生物学通报, 2004, 31（3）：36-41.
[14]孙迎雪, 沈丹丹, 杨哲, 等. 一株施氏假单胞菌Pseudomonas stutzeri DN-LWX19的脱氮性能[J]. 环境工程学报, 2015, 9（1）：247-252.
[15]弓湃, 王丽英, 尚立国, 等. 固氮施氏假单胞菌亚硝酸盐还原酶基因nirS转录特性及功能鉴定[J]. 微生物学通报, 2015, 42（1）：93-100.
[16]李献, 马光庭. 生物脱氮除硫（S_2-）菌株的分离和特征[J]. 广西农业生物科学, 2006, 25（1）：52-55.