Cloning and Expression Analysis of AmDREB2.1 in Ammopiptanthus mongolicus

doi:10.13560/j.cnki.biotech.bull.1985.2015.04.015

Abstract

Abstract: A new dehydration responsive element binding protein(DREB)transcription factor gene, which was named as AmDREB2.1, was isolated from Ammopiptanthus mongolicus(Masxim.)(Cheng f.)root transcriptome database, that it had been established in our previous work. The full length of the AmDREB2.1 cDNA was 978 bp, including a single 531 bp opening reading frame which encoded a 176-amino acid peptide with a conserved AP2 domain. Quantitative real-time PCR analysis revealed that the AmDREB2.1 expressed in leaf and root of A. mongolicus, but there were different expression patterns under drought or low temperature stress respectively, and it could be mainly induced by drought in root.

Key words: Ammopiptanthus mongolicus, AmDREB2.1, sequence analysis, expression pattern

Li Zhanglei, Gao Fei, Cao Yuzhen, Zhang Zhiwei, Wang Ning, Li Huayun, Zhou Yijun. Cloning and Expression Analysis of AmDREB2.1 in Ammopiptanthus mongolicus[J]. Biotechnology Bulletin, 2015, 31(3): 108-114.

References

[1] 潘伯荣, 葛学军. 我国沙冬青属植物保护生物学研究和保护实践的回顾与展望[A]. 中国生物多样性保护与研究进展VI—第六届全国生物多样性保护与持续利用研究会论文集(2004). 北京：气象出版社, 2005：373-392.
[2] Zhou YJ, Gao F, Liu R, et al. De novo sequencing and analysis of root transcriptome using 454 pyrosequencing to discover putative genes associated with drought tolerance in Ammopiptanthus mongolicus[J]. BMC Genomics, 2012, 13：266.
[3] Pang T, Ye CY, Xia X, et al. De novo sequencing and transcriptome analysis of the desert shrub, Ammopiptanthus mongolicus, during cold acclimation using Illumina/Solexa[J]. BMC Genomics, 2013, 14(1)：488.
[4] Guo H, Li Z, Zhou M, et al. cDNA-AFLP analysis reveals heat shock proteins play important roles in mediating cold, heat, and drought tolerance in Ammopiptanthus mongolicus[J]. Funct Integr Genomics, 2014, 14(1)：127-133.
[5] Wei Q, Kuai BK, Hu P, et al. Ectopic expression of an Ammopiptan-thus mongolicus H⁺-pyrophosphatase gene enhances drought and salt tolerance in Arabidopsis[J]. Plant Cell Tiss Organ Cult, 2012, 110：359-369.
[6] Deng LQ, Yu HQ, Liu YP, et al. Heterologous expression of antifreeze protein gene AnAFP from Ammopiptanthus nanus enhances cold tolerance in Escherichia coli and tobacco[J]. Gene, 2014, 539(1)：132-140.
[7] 李科友, 朱海兰. 植物非生物逆境胁迫DREB/CBF转录因子的研究进展[J]. 林业科学, 2011, 47(1)：124-134.
[8] Mizoi J, Shinozaki K, Yamaguchi-Shinozaki K. AP2 /ERF family transcription factors in plant abiotic stress responses[J]. Biochim Biophys Acta, 2012, 1819：86-96.
[9] Liu Q, Kasuga M, Sakuma Y, et al. Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought and low-temperature-responsive gene expression, respectively, in Arabidopsis[J]. Plant Cell, 1998, 10：391-406.
[10] Wang Q, Guan Y, Wu Y, et al. Overexpression of a rice OsDREB1F gene increases salt, drought, and low temperature tolerance in both Arabidopsis and rice[J]. Plant Mol Biol, 2008, 67：589-602.
[11] Qin F, Kakimoto M, Sakuma Y. Regulation and functional analysis of ZmDREB2A in response to drought and heat stresses in Zea mays L. [J]. Plant J, 2007, 50：54-69.
[12] 耿芳, 郭伟华, 郭玉双, 等. 烟草DREB转录因子新基因的克隆与功能分析[J]. 浙江大学学报：农业与生命科学版, 2011, 37(1)：22-30.
[13] Xu ZS, Chen M, Li LC, et al. Functions and application of the AP2 /ERF transcription factor family in crop improvement[J]. J Integr Plant Biol, 2011, 53(7)：570-585.
[14] 陈静, 高飞, 周宜君, 等. 改良Trizol法提取蒙古沙冬青总RNA[J]. 生物技术通报, 2013(10)：87-92.
[15] 杨杞, 白肖飞, 高阳, 等. 沙冬青CBF/DREB1转录因子cDNA的克隆及序列分析[J]. 基因组学与应用生物学, 2009, 28(6)：1043-1048.
[16] 张锋, 王学峰, 董博, 等. 沙冬青AmDREB3基因的克隆及植物表达载体构建[J]. 内蒙古农业大学学报, 2012(33)：5-6.
[17] Stockinger EJ, Gilmour SJ, Thomashow MF. Arabidopsis thaliana CBF1 encodes an AP2 domain containing transcription activator that binds to the C-repeat /DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit[J]. Proc Natl Acad Sci USA, 1997, 94：1035-1040．
[18] Lata C, Prasad M. Role of DREBs in regulation of abiotic stress responses in plants[J]. J Exp Bot, 2011, 62(14)：4731-4748.
[19] Li XP, Tian AG, Luo GZ, et al. Soybean DRE-binding transcription factors that are responsive to abiotic stresses[J]. Theor Appl Genet, 2005, 110(8)：1355-1362.
[20] Srivastav A, Mehta S, Lindlof A, et al. Over-represented promoter motifs in abiotic stress-induced DREB genes of rice and sorghum and their probable role in regulation of gene expression[J]. Plant Signal Behav, 2010, 5(7)：775-784.