Optimizing the Method of Acetone Powder to Extract Polyphenoloxidase from Honeysuckle Using Response Surface Methodology

doi:10.13560/j.cnki.biotech.bull.1985.2015.07.031

Abstract

Abstract: In this study, the method of acetone powder was optimized to extract the polyphenoloxidase(PPO)from honeysuckle flowers(Lonicera japonica)by response surface methodology. The results showed that three operating parameters had great effects on the PPO specific activity, and their effects were ranked as follows：extraction time > buffer pH > material-to-liquid ratio. The established regression model was highly fitting(R²= 99. 31%), and the interaction effect between buffer pH and material-to-liquid ratio was extremely significant. The optimal extraction condition was pH 7. 49, material-to-liquid ratio 1∶120 and extraction time 14. 5 min, under which the PPO specific activity was 137. 389 U/mg.

Key words: Lonicera japonica, polyphenoloxidase, acetone powder, extract, response surface

Qu Zheng, Luo Lei, Yang Bin, Kang Xinyan. Optimizing the Method of Acetone Powder to Extract Polyphenoloxidase from Honeysuckle Using Response Surface Methodology[J]. Biotechnology Bulletin, 2015, 31(7): 201-206.

References

[1] 武晓红, 田智勇, 王焕. 金银花的研究新进展[J]. 时珍国医国药, 2005, 16(12)：111-112.
[2] 李立祥, 吴红梅. 提取方法对茶多酚氧化酶的影响[J]. 中国茶叶加工, 2001, (4)：26-31.
[3] 田玉庭, 岳田利, 袁亚宏, 等. 澳洲青苹多酚氧化酶的分离纯化研究 [J]. 食品研究与开发, 2006, 127(5)：180-182.
[4] Ben-Shalom N, Kahn V, Harel E, et al. Catechol oxidase from green olivers[J]. Properties and Partial Purification Phytochem, 1997, (16)：1153-1158.
[5]施春华. 烟草多酚氧化酶的分离纯化, 光谱性质, 反应机理和应用研究[D]. 合肥：中国科学技术大学, 2002.
[6]He SL, Liu HX, Zhou SJ, et al. A Preliminary study on poly phenol oxidase extraction from sugarcane juice[C]. South China University of Technology(SCUT). Biennial World Conference on Recent Development in Sugar Technologies. Guangzhou：SCUT, 2009, 183-186.
[7]罗磊, 周燕燕, 刘云宏, 等. 响应面法优化金银花多酚氧化酶提取工艺[J]. 食品科学, 2014, 35(3)：117-121.
[8]张宽朝, 全明吉. 黑豆多酚氧化酶的分离纯化及其酶学性质的研究[J]. 中国饲料, 2012(19)：38-41.
[9]刘亮. 荔枝果皮多酚氧化酶内源底物的确定及其促褐变机制[D]. 武汉：华中农业大学, 2008.
[10]徐仁华. 蒜氨酸酶的分离纯化及其酶学性质研究[D]. 南昌：南昌大学, 2008.
[11]李玲. 砀山酥梨果实PPO酶学特性、PPO克隆及生物信息学分析[D]. 合肥：安徽农业大学, 2011.
[12] Kouakou TH, Kouadio YJ, Kouamé P, et al. Purification and biochemical characterization of polyphenol oxidases from embryogenic and nonembryogenic cotton(Gossypium hirsutum L.)cells[J]. Appl Biochem Biotechnol, 2009, 158：285-301.
[13]王磊. 马铃薯酶促褐变机理研究[D]. 南昌：南昌大学, 2012.
[14]Gao ZJ, Han XH, Xiao XG. Purification and characterisation of polyphenol oxidase from red Swiss chard[J]. Food Chemistry, 2009(117)：342-348.
[15]孟雅, 李刚, 崔焱, 张龙. 马铃薯多酚氧化酶的提取纯化条件对其活性影响的研究[J]. 化学与生物工程, 2006, 23(10)：47-49.
[16]刘文山, 肖凯军, 郭祀远. 苹果多酚氧化酶的提取及其抑制作用的研究[J]. 现代食品科技, 2006, 22(4)：82-84.
[17]王丽霞. 中华芦荟多酚氧化酶的分离纯化[J]. 淮阴师范学院学报：自然科学版, 2008, 7(4)：319-321.
[18]胡燕妮. 杨桃果实多酚氧化酶的研究[D]. 南宁：广西大学, 2008.