Cloning and Tissue-specific Expression of CAPN3 Gene in Yak

doi:10.13560/j.cnki.biotech.bull.1985.2016.01.025

Abstract

Abstract: Using the longissimus of yak(Bos grunniens)back as material, the CDs sequence of yak CAPN3 gene was cloned by RT-PCR, and it was analyzed by bioinformatics. The results indicated that the length of CDs in yak CAPN3 gene was 2 469 bp and encoding 822 amino acid residues. Bioinformatics analysis showed that the protein encoded by CAPN3 was the non-secretory surface protein, containing 35 phosphorylation site, and mainly played a biological role in the cytoplasm and nuclei. The secondary structure was mainly composed of α-helices, random coil, extended chain and β-turn, having the structure domains of CysPc, calpain-Ⅲ and EFh families and no signal peptide. The CAPN3 of yak was the most similar with those of Bos taurus, Ovis aries and Sus scrofa in phylogenetic tree. Real-time PCR analysis revealed the expressions of CAPN3 in varied tissues. There were expressions in all 7 tissues, and they were high in the muscle and pancreas.

Key words: Bos grunniens, CAPN3 gene, cloning, bioinformatics, expression pattern

WANG Ying-jie, YAN Ping, PAN He-ping, WU Xiao-yun, LI Ming-xia. Cloning and Tissue-specific Expression of CAPN3 Gene in Yak[J]. Biotechnology Bulletin, 2016, 32(1): 149-155.

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