Analysis of the DNA Methylation of SlGLD1 Regulated by JMJ524 in Tomato

Abstract

Abstract: This work is to reveal the mechanism of JMJ524 modulatinges the SlGLD1 expression by affecting the DNA methylation of SlGLD1，and leading to the dwarf of tomato insensitive to gibberellin. Bioinformatics and BSP（Bisulfite sequencing PCR）were employed to analyze the DNA methylation of SlGLD1 in inhibitory line and wild line of JMJ524. Results showed that the SlGLD1 promoter sequence was isolated，which contained 3 GA response cis-elements. The DNAs of SlGLD1 promoter and coding sequence were highly methylated，moreover，the methylation pattern varied at the different tissues and mutants. Two GC islands were found in this coding region of the gene，thus it was inferred that the methylation of SlGLD1 might happen in the region. Further，the analysis by BSP of the 2 GC islands indicated that the DNA CpG in the 2 regions of SlGLD1 was highly methylated. The whole methylation of DNA in the inhibitory line of JMJ524，especially CpG methylation was significantly lower than wild-type M82. The inhibited expression by JMJ524 resulted in the decrease of gene methylation of SlGLD1，therefore the expression of the gene was activated，which might be the one of factors causing the dwarf of tomato plant.

Key words: JmjC, GLD1, DNA methylation, tomato

CLC Number:

10.13560/j.cnki.biotech.bull.1985.2016.12.014

YANG Wei,PAN Yu,SU Cheng-gang,ZHANG Xing-guo,LI Jin-hua. Analysis of the DNA Methylation of SlGLD1 Regulated by JMJ524 in Tomato[J]. Biotechnology Bulletin, 2016, 32(12): 79-85.

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