Resistance Evaluation of Bt cry1Ah-transgenic Maize to Asian Corn Borer, Cotton Bollworm and Oriental Armyworm

doi:10.13560/j.cnki.biotech.bull.1985.2016.06.011

Abstract

Abstract: Bt cry1Ah gene was transferred into inbred line maize Zong31 via Agrobacterium-mediated method，and transgenic maize HGK60 with significant resistance to corn borer was acquired. In order to investigate its insecticidal activity to Lepidoptera pests，we evaluated the insecticidal effects of HGK60 to Asian corn boner(Ostrinia furnacalis)，cotton bollworm(Mythimna separate(Walker))and oriental armyworm(Helicoverpa armigera Hubne)through laboratory and field bioassay. The results of laboratory bioassay indicated that no Asian corn boner feeding on HGK60 leaves survived. HGK60 presented the toxic effect on neonate of cotton bollworm，and different tissues of it had different insecticidal effects. Compared to the non-transgenic maize，the body-weight of armyworm neonate was significantly inhibited after a week of feeding HGK 60 leaves. The results of field bioassay showed that HGK60 had solid insecticidal effects to O. furnacalis and H. armigera in high resistance level，while the efficacy to M. separate was in resistance level.

Key words: cry1Ah gene, maize, Ostrinia furnacalis, Helicoverpa armigera, Mythimna separate Hubne

SONG Miao, WANG Hai, ZHANG Jie, HE Kang-lai, LIANG Ge-mei, ZHU Li, HUANG Da-fang, LANG Zhi-hong. Resistance Evaluation of Bt cry1Ah-transgenic Maize to Asian Corn Borer, Cotton Bollworm and Oriental Armyworm[J]. Biotechnology Bulletin, 2016, 32(6): 69-75.

References

[1] 张洪刚, 鲁新, 何康来, 等. 亚洲玉米螟抗寒及低温生存对策[J]. 植物保护学报, 2010, 37(5)：398-402.
[2]常雪艳, 何康来, 王振营, 等. 转Bt基因玉米对棉铃虫的抗性评价[J]. 植物保护学报, 2006, 33(4)：347-378.
[3]宋立秋, 石洁, 王振营, 等. 亚洲玉米螟为害对玉米镰孢穗腐病发生程度的影响[J]. 植物保护, 2012, 38(6)：50-53, 58.
[4]李人杰, 魏铁松, 郭聪聪, 等. 棉铃虫为害与拟轮生镰孢侵染对玉米穗腐病发生及玉米籽粒中伏马毒素污染水平的影响[J]. 植物保护, 2015, 41(4)：68-72.
[5]段灿星, 王晓鸣, 宋凤景, 等. 玉米抗穗腐病研究进展[J]. 中国农业科学, 2015, 11(48)：2152-2164.
[6]常雪, 常雪艳, 何康来, 等. 转cry1Ab基因玉米对黏虫的抗性评价[J]. 植物保护学报, 2007, 34(3)：225-228.
[7]Clive J. ISAAA Report on global status of biotech/GM crops：2013[J]. China Biotechnology, 2014, 34(1)：1-8.
[8]Gordon-kamm WJ, Spencer TM, Mangano ML, et al. Transformation of maize cells and regeneration of fertile transgenic plants[J]. Plant Cell, 1990, 2(7)：603-618.
[9]Denolf P, Hendrickx K, Van Damme J, et al. Cloning and characterization of Manduca sexta and Plutella xylostella midgut aminopeptidase N enzymes related to Bacillus thuringiensiis toxin-binding proteins[J]. Eur J Biochem, 1997, 248(3)：748-761.
[10]Xue J, Liang GM, Crickmore N, et al. Cloning and characterization of a novel Cry1A toxin from Bacillus thuringiensis with high toxicity to the Asian corn borer and other lepidopteran insects[J]. FEMS Microbiology Letters, 2008, 280(1)：95-101.
[11]Xue J, Zhou ZS, Song FP, et al. Identification of the minimal active fragment of the Cry1Ahtoxin[J]. Biotechnology Letters, 2011, 33(3)：531-537.
[12]Rasheda J, Muhammad SK, Yusuf Z, et al. Codon optimization of cry1Ab gene for hyper expression in plant organelles[J]. Molecular Biology Reports, 2010, 37(2)：1011-1017.
[13 Li XY, Lang ZH, Zhang J, et al. Acquisition of insect-resistant transgenic maize harboring a truncated crylAh gene via Agrobacterium-mediated transformation[J]. Journal of Integrative Agriculture, 2014, 13(5)：937-944.
[14]戴军, 李秀影, 朱莉, 等. 转Bt cry1Ah基因抗虫玉米的分子检测及农艺性状分析[J]. 生物技术通报, 2014(5)：62-68.
[15]岳同卿, 郎志宏, 王延峰, 等. 转Bt cry1Ah基因抗虫玉米的获得及其遗传稳定性分析[J]. 农业生物技术学报, 2010, 18(4)：638-644.
[16]王冬妍, 王振营, 何康来, 等. Bt玉米杀虫蛋白含量的时空表达及对亚洲玉米螟的杀虫效果[J]. 中国农业科学, 2004, 37(8)：1155-1159.
[17]芮昌辉, 范贤林, 孟香清, 等. 转Bt基因抗虫玉米对棉铃虫杀虫活性的初步评价[J]. 农药学学报, 2000, 2(3)：88-90.
[18]邰桂玲, 邓曙东, 张青文, 等. Bt玉米(MG95)对黏虫的抗性和拒食作用[J]. 昆虫知识, 2004, 41(5)：422-426.
[19]王振营, 王冬妍, 何康来, 等. 转Bt基因玉米对黏虫的室内杀虫效果评价[J]. 植物保护学报, 2005, 32(2)：153-157.
[20]郭井菲. 玉米茎秆机械强度与抗玉米螟钻蛀为害的关系[D]. 北京：中国农业科学院, 2014.
[21] 杨芳兰. 玉米田棉铃虫药剂防治试验效果[J]. 甘肃农业科技, 2002, 7：45-46.
[22]刘杰, 姜玉英, 曾娟. 2013年我国黏虫发生特点分析[J]. 植物保护, 2015, 3(41)：131-137.
[23]宋萍. Bt Cry1Ac毒蛋白对棉铃虫和黏虫作用机理研究[D]. 保定：河北农业大学, 2003.
[24]Krogh PH, Griffiths B, Dem?ar D, et al. Responses by earthworms to reduced tillage in herbicide tolerant maize and Bt maize cropping systems[J]. Pedobiologia, 2007, 51(3)：219-227.
[25]王冬妍, 王振营, 何康来, 等. 转Bt基因抗虫玉米在害虫综合治理中的作用及生态风险[J]. 植物保护学报, 2003, 30(1)：97-106.
[26]陆宴辉, 梁革梅. Bt作物系统害虫发生演替研究进展[J]. 植物保护, 2016, 1(42)：7-11.
[27]陆宴辉. Bt棉花害虫综合治理研究前沿[J]. 应用昆虫学报, 2012, 4(49)：809-819.
[28]林珠凤, 陈海燕, 吉训聪. 害虫对转Bt基因作物的抗性及治理[J]. 热带生物学报, 2015, 4(6)：497-503.

[1]	WANG Bao-bao, WANG Hai-yang. Molecular Design of Ideal Plant Architecture for High-density Tolerance of Maize Plant [J]. Biotechnology Bulletin, 2023, 39(8): 11-30.
[2]	ZHANG Dao-lei, GAN Yu-jun, LE Liang, PU Li. Epigenetic Regulation of Yield-related Traits in Maize and Epibreeding [J]. Biotechnology Bulletin, 2023, 39(8): 31-42.
[3]	LENG Yan, MA Xiao-wei, CHEN Guang, REN He, LI Xiang. High-yield Contests in Maize Facilitate the Vitalization of China’s Seed Industry [J]. Biotechnology Bulletin, 2023, 39(8): 4-10.
[4]	WANG Tian-yi, WANG Rong-huan, WANG Xia-qing, ZHANG Ru-yang, XU Rui-bin, JIAO Yan-yan, SUN Xuan, WANG Ji-dong, SONG Wei, ZHAO Jiu-ran. Research in Maize Dwarf Genes and Dwarf Breeding [J]. Biotechnology Bulletin, 2023, 39(8): 43-51.
[5]	LIU Yue-e, XU Tian-jun, CAI Wan-tao, LYU Tian-fang, ZHANG Yong, XUE Hong-he, WANG Rong-huan, ZHAO Jiu-ran. Current Status and Prospects of Maize Super High Yield Research in China [J]. Biotechnology Bulletin, 2023, 39(8): 52-61.
[6]	ZHU Shao-xi, JIN Zhao-yang, GE Jian-rong, WANG Rui, WANG Feng-ge, LU Yun-cai. High-throughput Specific Detection Methods for Transgenic Maize Based on the KASP Platform [J]. Biotechnology Bulletin, 2023, 39(6): 133-140.
[7]	CHEN Nan-nan, WANG Chun-lai, JIANG Zhen-zhong, JIAO Peng, GUAN Shu-yan, MA Yi-yong. Genetic Transformation and Chilling Resistance Analysis of Maize ZmDHN15 Gene in Tobacco [J]. Biotechnology Bulletin, 2023, 39(4): 259-267.
[8]	LI Sheng-yan, LI Xiang-yin, LI Peng-cheng, ZHANG Ming-jun, ZHANG Jie, LANG Zhi-hong. Identification of Target Traits and Genetic Stability of Transgenic Maize 2HVB5 [J]. Biotechnology Bulletin, 2023, 39(1): 21-30.
[9]	LI Dong-yang, XIAO Bing, WANG Chen-yao, YANG Xian-ming, LIANG Jin-gang, WU Kong-ming. Spatio-temporal Expression of Cry1Ab/Cry2Aj Insecticidal Protein in Genetically Modified Maize Ruifeng 125 with Stacked Insect and Herbicide Resistance Traits [J]. Biotechnology Bulletin, 2023, 39(1): 31-39.
[10]	LI Peng-cheng, ZHANG Ming-jun, WANG Yin-xiao, LI Xiang-yin, LI Sheng-yan, LANG Zhi-hong. Insect Resistance Identification and Agronomy Traits Analysis of Transgenic Maize HGK60 with Different Genetic Backgrounds [J]. Biotechnology Bulletin, 2023, 39(1): 40-47.
[11]	JIN Yun-qian, WANG Bin, GUO Shu-lei, ZHAO Lin-xi, HAN Zan-ping. Research Progress in Gibberellin Regulation on Maize Seed Vigor [J]. Biotechnology Bulletin, 2023, 39(1): 84-94.
[12]	ZHU Jing, YU Cun. Effects of Trichoderma longibrachiatum on Maize Growth,Soil Fertility and Rhizosphere Microorganism [J]. Biotechnology Bulletin, 2022, 38(4): 230-241.
[13]	ZHANG Qian, XU Chun-yan, ZHANG Duo, WANG Ya-hui, LIANG Xin-ying, LI Hui. Isolation of Maize Straw-decomposing Bacteria in Yellow-cinnamon Soil and Its Ability of Promoting Decomposition [J]. Biotechnology Bulletin, 2022, 38(12): 233-243.
[14]	ZHANG Tong-tong, ZHENG Deng-yu, WU Zhong-yi, ZHANG Zhong-bao, YU Rong. Functional Analysis of ZmNF-YB13 Responding to Drought and Salt Stress [J]. Biotechnology Bulletin, 2022, 38(10): 115-123.
[15]	JIAO Yue, HAN Yu, YANG Qiao, HUANG Yao-hui, AN Ji-cui, YANG Ya-zhou, YE Ji-ming. Commercialization Development Trend of Genetically Modified Maize and the Enlightenment [J]. Biotechnology Bulletin, 2021, 37(4): 164-176.