Expression Profile Analysis of Maize Resistance Under Osmotic Stress Induced by Secondary Metabolites of Endophytes

doi:10.13560/j.cnki.biotech.bull.1985.2016.07.013

Abstract

Abstract: Genechip technology was used to analyze the expression profile of differential genes in corn treated by metabolites of endophyte under 10% PEG osmotic stress. The results showed that there were 441 differentially expressed genes identified in this study including 147 up-regulated genes and 294 down-regulated genes,participating in 21 metabolic pathways. The genes with differential expressions were classified by GO-ranking methods through CapitalBio Molecule Annotation System V 3.0（MAS）. Among them 45% participated in various biological processes,18% were related to synthesis of cellular components,the coded products of 36% genes were related to performing a variety of molecular function,and 1% corresponded with other unknown sequences in GenBank. Thus the role of these genes still needs further study. Pathways analysis using the KEGG pathway database showed the differentially expressed genes broadly were involved in basic metabolism,energy metabolism,secondary metabolism and signal transduction pathways. The expression profile analysis indicate that the effects of secondary metabolites on corn under osmotic stress are synergistical processes by many genes in many pathways.

Key words: endophyte, maize, osmotic stress, gene expression profile

WANG Na, GONG Na, LIU Guo-li, MA Xiao-ying, YANG Zhen, YANG Tao. Expression Profile Analysis of Maize Resistance Under Osmotic Stress Induced by Secondary Metabolites of Endophytes[J]. Biotechnology Bulletin, 2016, 32(7): 87-92.

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