Research on Population Structures and Distribution Characteristics of Indigenous Microorganisms in Bohai Oil Field N

doi:10.13560/j.cnki.biotech.bull.1985.2016.09.012

Abstract

Abstract: Denaturing gradient gel electrophoresis（DGGE）method was used to analyze the structures of microorganism population in water injection wells and no-water injection wells of an oil reservoir in Bohai oil field N,and investigate the process effect of water injection to the abundance and population of microbial communities in the reservoir,aiming at providing technical support for the field test of indigenous microorganism enhanced oil recovery. The results showed that bacterial abundance and species in water injection wells were significantly higher than that in no-water injection wells,main bacteria in water injection well were Azospira sp.,Pseudomonas sp.,Thauera sp.. The species and abundance of archaea in water injection wells also presented differences from no-water injection wells,main archaea were Methanothermobacter thermautotrophicus,Methanosaeta,and Crenarchaeote. Species distribution of bacteria and archaea in water injection wells and no-water injection wells were limited,but their abundances were high,and which were mainly beneficial bacteria increasing oil recovery,indicating that this block possesses the condition to implement the microbial enhanced oil recovery technology by indigenous microorganisms.

Key words: water injection oil reservoir, denaturing gradient gel electrophoresis, bacteria, archaea

WANG Da-wei¹, ZHANG Jian¹, HE Chun-bai¹, MA Ting², LÜ Xin¹, LI Qiang¹. Research on Population Structures and Distribution Characteristics of Indigenous Microorganisms in Bohai Oil Field N[J]. Biotechnology Bulletin, 2016, 32(9): 83-92.

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