Research Advances on Colonization of Plant Growth-promoting Rhizobacteria

doi:10.13560/j.cnki.biotech.bull.1985.2017.02.002

Abstract

Abstract: The PGPB（plant growth-promoting bacterium）presents a promising prospect in agriculture due to its advantages such as inhibiting rhizosphere pathogens,promoting plant growth,and increasing crop production. This paper first summarizes the species of plant growth-promoting rhizobacteria and the promoting mechanisms of involved colonization,and demonstrates the colonization process of rhizobacteria as well as the factors influencing the colonization of bacteria in rhizosphere. Then the paper reviews the detection methods of microorganisms in the colonization of bacteria,including antibiotics marking,immunological methods,and exogenous gene marking methods. Further,the paper discusses high-throughput sequencing technologies widely used in the rhizosphere colonization. Finally,it is concluded that the future researches on interpreting strains in genetic level,gaining microbial community diversity in plant rhizosphere and the functional genes should be of significance to better predict the interaction of PGPB and its host plant,and to apply bio-fertilizers in field.

Key words: colonization, rhizobacteria, growth-promoting, high-throughput sequencing

SUN Zhen, ZHENG Liang, QIU Hao-bin. Research Advances on Colonization of Plant Growth-promoting Rhizobacteria[J]. Biotechnology Bulletin, 2017, 33(2): 8-15.

References

[1] Bhattacharyya PN, Jha DK. Plant growth-promoting rhizobacteria（PGPR）：emergence in agriculture[J]. World Journal of Microbiology Biotechnology, 2012, 28（4）：1327-1350.
[2] Luna MF, Galar ML, Aprea J, et al. Colonization of sorghum and wheat by seed inoculation with Gluconacetobacter diazotrophicus[J]. Biotechnology Letters, 2010, 32（8）：1071-1076.
[3] Shankar M, Ponraj P, Llakkiam D, et al. Root colonization of a rice growth promoting strain of Enterobacter cloacae[J]. Journal of Basic Microbiology, 2011, 51（5）：523-530.
[4] Cindy HW, Stéphanie MB, Gary LA, et al. Developing microbe-plant interactions for applications in plant-growth promotion and disease control, production of useful compounds, remediation and carbon sequestration[J]. Microbial Biotechnology, 2009, 2（4）：428-440.
[5] Compant S, Clement C, Sessitsch A. Plant growth-promoting bacteria in the rhizo- and endosphere of plants：Their role, colonization, mechanisms involved and prospects for utilization[J]. Soil Biology Biochemistry, 2010, 42（5）：669-678.
[6] 刘邮洲, 梁雪杰, 乔俊卿, 等. 枯草芽孢杆菌PTS-394的GFP标记及定殖能力[J]. 植物保护学报, 2014, 41（4）：416-421.
[7] 黎志坤, 朱红惠. 一株番茄青枯病生防菌的鉴定与防病、定殖能力[J]. 微生物学报, 2010, 50（3）：342-349.
[8] Rudrappa T, Czymmek KJ, Pare PW, et al. Root-secreted malic acid recruits beneficial soil bacteria[J]. Plant Physiol, 2008, 148（3）：1547-1556.
[9] Liu XM, Zhao HX, Chen SF. Colonization of maize and rice plants by strain Bacillus megaterium C4[J]. Current Microbiology, 2005, 52：186-190.
[10] Yuan J, Zhang N, Huang Q, et al. Organic acids from root exudates of banana help root colonization of PGPR strain Bacillus amyloliquefaciens NJN-6[J]. Scientific Reports, 2015, 5：13438.
[11] 张冬冬, 刘涛, 高同国, 等. 棉花黄萎病拮抗细菌Z-5菌株的定殖能力检测[J]. 棉花学报, 2013, 25（6）：510-516.
[12] Farag MA, Zhang H, Ryu CM. Dynamic chemical communication between plants and bacteria through airborne signals：induced resistance by bacterial volatiles[J]. Journal of Microbiology, 50（3）：380-385.
[13] Rahi MU, Arundhathi, Reddy G. Bacillus cereus and Enterobacter cancerogenus screened for their efficient plant growth promoting traits rhizobacteria（PGPR）and antagonistic traits among sixteen bacterial isolated from rhizospheric soils ofPigeon pea[J]. African Journal of Microbiology Research, 2011, 5（15）：2090-2094.
[14] Zhao J, Zhou L, Wub J. Promotion of Salvia miltiorrhiza hairy root growth and tanshinone production by polysaccharide-protein fractions of plant growth-promoting rhizobacterium Bacillus cereus[J]. Process Biochemistry, 2010, 45：1517-1522.
[15] Maleki M, Mostafaee S, Mokhtarnejad L, et al. Characterization of Pseudomonas fluorescens strain CV6 isolated from cucumber rhizosphere in Varamin as a potential biocontrol agent[J]. Australian Journal of Crop Science, 2010, 4（9）：676-683.
[16] Gamalero E, Lingua G, Tombolini R, et al. Colonization of tomato root seedling by Pseudomonas fluorescens 92rkG5：Spatio-temporal dynamics, localization, organization, viability, and culturability[J]. Microbial Ecology, 2005, 50：2, 289-297.
[17] Siddiqui IA, Shaukat SS, Khan GH, et al. Suppression of Meloidogyne javanica by Pseudomonas aeruginosa IE-6S + in tomato：the influence of NaCl, oxygen and iron levels[J]. Soil Biology and Biochemistry, 2003, 35（12）：1625-1634.
18]Buddrus-Schiemann K, Schmid M, Schreiner K, et al. Root colonization by Pseudomonas sp. DSMZ13134 and impact on the indigenous rhizosphere bacterial community of barley[J]. Microbiology Ecology, 2010, 60（2）：381-393.
[19] Ghorbanpour M, Hosseini S, Rezazadeh M, et al. Hyoscyamine and scopolamine production of black henbane（Hyoscyamus niger）infected with Pseudomonas putida and P. fluorescens strains under water deficit stress[J]. Planta Medica, 2010, 76（12）：167.
[20] Chen F, Guo YB, Wang JH, et al. Biological control of grape crown gall by Rahnella aquatilis HX2[J]. Plant Disease, 2007, 91（8）：957-963.
[21] 郭岩彬, 吴文良, 孙真, 等. 滋养节杆菌Ar13及其作为植物根际促生细菌的应用：中国, 201210103736. 2[P]. 2012-08-22.
[22] Ambrosini A, Beneduzi A, Stefanski T, et al. Screening of plant growth promoting Rhizobacteria isolated from sunflower（Helianthus annuus L.）[J]. Plant and Soil, 2012, 356（1）：245-264.
[23] Liu Y, Wang H, Sun XL, et al. Study on Mechanisms of colonization of Nitrogen-Fixing PGPB, Klebsiella pneumonia NG14 on the root surface of rice and the formation of biofilm[J]. Current Microbiology, 2011, 62：1113-1122.
[24] Rothballer M, Eckert B, Schmid M, et al. Endophytic root coloniza-tion of gramineous plants by Herbaspirillum frisingense[J]. FEMS Microbiology Ecology, 2008, 66：85-95.
[25] Baldotto LEB, Olivares FL, Bressan-Smith R. Structural interaction between gfp-labeled diazotrophic endophic bacterium Herbaspirillum seropedicae RAM10 and pineapple plantlets vitoria[J]. Brazillian Journal of Microbiology, 2011, 42：114-125.
[26] Figueiredo MVB, Burity HA, Martinez CR, et al. Alleviation of drought stress in the common bean（Phaseolus vulgaris L.）by co-inoculation with Paenibacillus polymyxa and Rhizobium tropici[J]. Applied Soil Ecology, 2008, 40（1）：182-188.
[27] Boehm M, Hurek T, Reinhold HB. Twitching motility is essential for endophytic rice colonization by the N-2-fixing endophyte Azoarcus sp. strain BH72[J]. Molecular Plant-Microbe Interactions, 2007, 20（5）：526-533.
[28] Joe MM, Jaleel CA, Sivakumar PK, et al. Co-aggregation in Azospirillum brasilensense MTCC-125 with other PGPR strains：effect of physical and chemical factors and stress endurance ability[J]. Journal of Taiwan Institute of Chemical Engineers, 2009, 40（5）：491-499.
[29] Sumayo M, Hahm MS, Ghim SY, et al. Determinants of plant growth-promoting Ochrobactrum lupini KUDC1013 involved in induction of systemic resistance against Pectobacterium carotovorum subsp carotovorum in tobacco leaves[J]. Plant Pathology Journal, 2013, 29（2）：174-181.
[30] Cho HS. Park SY, Ryu CM, et al. Interference of quorum sensing and virulence of the rice pathogen Burkholderia glumae by an engineered endophytic bacterium[J]. FEMS Microbiology Ecology, 2007, 60（1）：14-23.
[31] Ahemad M, Kibret M. Mechanisms and applications of plant growth promoting rhizobacteria：current perspective[J]. Journal of King Saud University：Science, 2014, 26（1）：1-20.
[32] Spaepen S, Vanderleyden J. Auxin and plant-microbe interactions [J]. Cold Spring Harbor Perspectives in Biology, 2011, 3（4）：a001438.
[33] Pablo RH, Leo SVO, Jan DVE. Properties of bacterial endophytes and their proposed role in plant growth[J]. Trends in Microbiology, 2008, 16：463-471.
[34] Guo YB, Li JY, Li L, et al. Mutations that disrupt either the pqq or the gdh gene of Rahnella aquatilis abolish the production of an antibacterial substance and result in reduced biological control of grapevine crown gall[J]. Applied and Environmental Microbiology, 2009, 75（21）：6792-6803.
[35] Chauhan H, Bagyaraj DJ, Selvakumar G, et al. Novel plant growth promoting rhizobacteria-prospects and potential[J]. Applied Soil Ecology, 2015, 95：38-53.
[36] Lugtenberg B, Kamilova F. Plant-Growth-Promoting Rhizobacteria [J]. Annual Review Microbiology, 2009. 63：541-556.
[37] Shephard RW, Lindow S. Two dissimilar N-acyl-Homoserine lactone acylases of Pseudomonas syringae influence colony and biofilm morphology[J]. Applied Environment Microbiology, 2009, 75（1）：45-53.
[38] 龙良鲲, 肖崇刚. 内生细菌01-144在番茄根茎内定殖的初步研究[J]. 微生物学通报, 2003, 30（5）：53-56.
[39] 孙真. 拉恩氏水生菌（Rahnella aquatilis）HX2在玉米根系的定殖研究[D]. 北京：中国农业大学, 2010.
[40] 刘纯, 黄红娟, 张朝贤, 等. 假高粱根系分泌物对土壤细菌群落多样性的影响[J]. 生态环境学报, 2013, 22（7）：1124-1128.
[41] Hardoim PR, van Overbeek S, van Elsas JD. Properties of bacterial endophytes and their proposed role in plant growth[J]. Trends in Microbiology, 2008, 16（10）：463-471.
[42] Watt M, McCully ME, Kirkegaard JA. Soil strength and rate of root elongation alter the accumulation of Pseudomonas spp. and other bacteria in the rhizosphere of wheat[J]. Functional Plant Biology, 2003, 30（5）：483-491.
[43] 年洪娟, 陈丽梅. 土壤有益细菌在植物根际竞争定殖的影响因素[J]. 生态学杂志, 2010, 29（6）：1235-1239.
[44] van Loon LC. Plant responses to plant growth-promoting bacteria[J]. European Journal of Plant Pathology, 2007, 119：243-254.
[45] Dashti NH, Ali NY, Cherian VM, et al. Application of plant growth-promoting rhizobacteria（PGPR）in combination with a mild strain of cucumber virus（CMV）associated with viral satellite RNAs to enhance growth and protection against a virulent strain of CMV in tomato[J]. Canadian Journal of Plant Pathology-Revue Canadienne de Phytopathologie, 2012, 34（2）：177-186.
[46] Krzyanowska D, Obuchowski M, Bikowski M, et al. Colonization of potato rhizosphere by GFP-Tagged Bacillus subtilis MB73/2, Pseudomonas sp. P482 and Ochrobactrum sp. A44 shown on large sections of roots using enrichment sample preparation and confocal laser scanning microscopy[J]. Sensors, 2012, 12（12）：17608-17619.
[47] Teixeira LC, Peixoto RS, Cury JC, et al. Bacterial diversity in rhizosphere soil from Antarctic vascular plants of Admiralty Bay, maritime Antarctica[J]. The ISME Journal, 2010, 4（8）：989-1001.
[48] Qiu MH, Zhang RF, Xue C, et al. Application of bio-organic fertilizer can control Fusarium wilt of cucumber plants by regulating microbial community of rhizosphere soil[J]. Biology and Fertility of Soils, 2012, 48（7）：807-816.
[49] Allen HK, Moe LA, Rodbumrer J, et al. Functional metagenomics reveals diverse beta-lactamases in a remote Alaskan soil[J]. ISME J, 2009, 3（2）：243-251.
[50] Dong L, Meng Y, Wang J, et al. Evaluation of droplet digital PCR for characterizing plasmid reference material used for quantifying ammonia oxidizers and denitrifiers[J]. Analytical and Bioanalytical Chemistry, 2014, 406（6）：1701-1712.
[51] Rosch C, Mergel A, Bothe H. Biodiversity of denitrifying and dinitrogen-fixing bacteria in an acid forest soil[J]. Applied Environmental Microbiology, 2002, 68（8）：3818-3829.
[52] Zhang N, Yang DQ, Wang DD, et al. Whole transcriptomic analysis of the plant-beneficial rhizobacterium Bacillus amyloliquefaciens SQR9 during enhanced biofilm formation regulated by maize root exudates[J]. BMC Genomics, 2015, 16：685.