Cloning and Expression Profile of Interleukin-1β Gene in Schizothorax prenanti

doi:10.13560/j.cnki.biotech.bull.1985.2017.02.014

Abstract

Abstract: This work aims to characterize interleukin-1β（IL-1β）gene of Schizothorax prenanti. First,RT-PCR was used to amplify IL-1β gene with cDNA of S. prenanti spleen as template. Then,the IL-1β gene sequence was analyzed with various bioinformatics tools,and the expressions in 6 tissues of S. prenanti were detected used RT-PCR. As results,the length of IL-1β gene was 1 252 bp,and it encoded 276 amino acids. Its GenBank accession number was KU886235,the molecular weight of IL-1β was 31.25 kD,and pI was 5.45. It was induced as a hydrophilic protein,and its secondary structure mainly consisted of random coil and extended strand. There were 4 N-glycosylation sites and 16 phosphorylation sites in this protein. Phylogenetic relationships revealed that S. prenanti presented a closest genetic relationship with Cyprinus carpio and Carassius auratus. The analysis of tissue expression indicated that IL-1β gene was mainly expressed in the spleen and kidney. It provides theoretical evidence for further studying the biological function of IL-1β gene.

Key words: Schizothorax prenanti, interleukin-1β, cloning, protein analysis, gene expression

DUAN Hui-qin, WANG Li. Cloning and Expression Profile of Interleukin-1β Gene in Schizothorax prenanti[J]. Biotechnology Bulletin, 2017, 33(2): 97-101.

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