Inhibition of Brominated Furanone to Quorum Sensing Regulating Behaviors of Vibrio anguillarum

doi:10.13560/j.cnki.biotech.bull.1985.2017.04.030

Abstract

Abstract: The present study aims to investigate the inhibition effect of a quorum sensing（QS）inhibitor,brominated furanone（BMF）,on the QS-related genes and pathogenic factor of Vibrio anguillarum. The effects of BMF on the expressions of QS-related genes（vanI/R and luxS）were determined by RT-PCR at sub-minimal inhibitory concentration（MIC）. The role of BMF in the formation of biofilm and flagellum was observed by flagellum staining,and the role of BMF in the extracellular enzyme were detected by plate diffusion method. The results indicated that BMF with sub-MIC significantly suppressed the expression of vanI/R and luxS,reduced the capacity of V. anguillarum secreting protease and gelatinase,and inhibited the growth of flagellum and formation of biofilm,i.e.,the alleviated its moving ability.

Key words: brominated furanone, Vibrio anguillarum, quorum sensing, flagellum, extracellular enzyme, biofilm

PAN Yu-rong, ZHANG Cai-li, ZHU Su-qin, ZENG Ming-yong. Inhibition of Brominated Furanone to Quorum Sensing Regulating Behaviors of Vibrio anguillarum[J]. Biotechnology Bulletin, 2017, 33(4): 231-237.

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