Biotechnology Bulletin ›› 2017, Vol. 33 ›› Issue (8): 46-50.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0132

• Research Report • Previous Articles     Next Articles

Mapping-based Cloning of a Wax Crystal-Sparse Leaf Mutant wcl1 in Rice

WANG Jia-mei, AN Xue-jiao, ZHANG Zhi-guo   

  1. Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081
  • Received:2017-02-27 Online:2017-08-01 Published:2017-08-01

Abstract: Here a wax crystal-sparse leaf mutant(wcl1)was screened from rice T-DNA Insertion Mutant Library. The mutant wcl1has two main features as following:1)the wcl1 leaf cuticular wax reduced;2)the drought sensitivity in the wcl1 plant was higher than wild type. Map-based cloning of wcl1 revealed that a point mutation of G to T in the 10th exonof gene LOC_OS09g25850encodingketoacyl coenzymeA resulted in the termination of transcription. The analysisshowed that the wcl1was allelic to wsl2.

Key words: rice, wax crystal-sparse leaf mutant, map-based cloning