Cloning and Expression Analysis of PwNAC42 in Picea wilsonii

doi:10.13560/j.cnki.biotech.bull.1985.2017-0924

Abstract

Abstract: As one of the largest plant transcription factors，NAC is widely involved in growth and development，as well as the responses of plants to abiotic stresses. In this study，PwNAC42 cDNA was obtained by Picea wilsonii transcriptome data，its protein sequence was analyzed by bioinformatics，and the expression of PwNAC42 was detected by real-time qPCR. The results showed that the full-length of PwNAC42 cDNA was 1749 bp，including 1140 bp of ORF that encoded 379 amino acids. The molecular weight of PwNAC42 was 42.92 kD and the pI value was 6.53. PwNAC42 had phosphorylation sites of serine，threonine and tyrosine. It was a hydrophilic protein that had no signal peptide domain and transmembrane domain. RT-qPCR results showed that PwNAC42 mainly expressed in cones. At the same time，the expression of PwNAC42 under ABA，NaCl，drought and low temperature treatment changed significantly. Therefore，P. wilsonii PwNAC42 may play a role in cone development and response to abiotic stress.

Key words: Picea wilsonii, NAC transcription factor, PwNAC42, stress response

YUAN Yi-hang, ZHANG He-hua, YOU Han-li, ZHANG Ling-yun. Cloning and Expression Analysis of PwNAC42 in Picea wilsonii[J]. Biotechnology Bulletin, 2018, 34(3): 113-120.

References

［1］ Singh KB, et al. Transcription factors in plant defense and stress responses［J］. Curr Opin Plant Biol, 2002, 5（5）：430-436.
［2］Aida M, Ishida T, Fukaki H, et al. Genes involved in organ separation in Arabidopsis：an analysis of the cup-shaped cotyledon mutant［J］. The Plant Cell, 1997, 9（6）：841-857.
［3］Shen H, Yin Y, Chen F, et al. A bioinformatic analysis of NAC genes for plant cell wall development in relation to lignocellulosic bioenergy production［J］. Bioenergy Research, 2009, 2（4）：217-232.
［4］Ooka H, Satoh K, Doi K, et al. Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana［J］. DNA Research, 2003, 10（6）：239-247.
［5］Wang Z, Dane F. NAC（NAM/ATAF/CUC）transcription factors in different stresses and their signaling pathway［J］. Acta Physiologiae Plantarum, 2013, 35（5）：1397-1408.
［6］Olsen AN, Ernst HA, Leggio LL, et al. NAC transcription factors：structurally distinct, functionally diverse［J］. Trends Plant Sci, 2005, 10（2）：79-87.
［7］Rauf M, Arif M, Dortay H, et al. ORE1 balances leaf senescence against maintenance by antagonizing G2-like-mediated transcription［J］. Embo Reports, 2013, 14（4）：382-388.
［8］Shan W, Kuang J, Chen L, et al. Molecular characterization of banana NAC transcription factors and their interactions with ethylene signalling component EIL during fruit ripening［J］. J Exp Bot, 2012, 63（14）：5171-5187.
［9］Guan Q, Yue X, Zeng H, et al. The protein phosphatase RCF2 and its interacting partner NAC019 are critical for heat stress-responsive gene regulation and thermotolerance in Arabidopsis［J］. Plant Cell, 2014, 26（1）：438-453.
［10］ Jiang G, Jiang X, Lü P, et al. The rose（Rosa hybrida）NAC trans-cription factor 3 gene, RhNAC3, involved in ABA signaling path-way both in rose and Arabidopsis［J］. PLoS One, 2014, 9（10）：e109415.
［11］Mao X, Zhang H, et al. TaNAC2, a NAC-type wheat transcription factor conferring enhanced multiple abiotic stress tolerances in Arabidopsis［J］. J Exp Bot, 2012, 63（8）：2933-2946.
［12］张盾, 刘亚静, 李长江, 等. 青杄均一化cDNA文库构建及EST序列分析［J］. 生物技术通报, 2012（6）：71-76.
［13］Yu Y, Li Y, Huang G, et al. PwHAP5, a CCAAT-binding transcription factor, interacts with PwFKBP12 and plays a role in pollen tube growth orientation in Picea wilsonii［J］. J Exp Bot, 2011, 62（14）：4805-4817.
［14］周燕妮, 李艳芳, 张通, 等. 青杄PwUSP2基因的克隆和表达分析［J］. 植物生理学报, 2015, 51（8）：1307-1314.
［15］Souer E, Houwelingen AV, Kloos D, et al. The no apical meristem gene of petunia is required for pattern formation in embryos and flowers and is expressed at meristem and primordia boundaries［J］. Cell, 1996, 85（2）：159-170.
［16］Puranik S, Sahu PP, Srivastava PS, et al. NAC proteins：regulation and role in stress tolerance［J］. Trends Plant Sci, 2012, 17（6）：369-381.
［17］Ma J, Wang F, Li MY, et al. Genome wide analysis of the NAC transcription factor family in Chinese cabbage to elucidate responses to temperature stress［J］. Scientia Horticulturae, 2014, 165（3）：82-90.
［18］韩芹芹. 番茄SlNAC3基因通过延迟果实软化和改变类胡萝卜素的合成调控果实成熟［D］. 武汉：华中农业大学, 2011.
［19］Jeong JS, Kim YS, et al. Root-specific expression of OsNAC10 improves drought tolerance and grain yield in rice under field drought conditions［J］. Plant Physiol, 2010, 153（1）：185-197.
［20］Lu M, Ying S, Zhang DF, et al. A maize stress-responsive NAC transcription factor, ZmSNAC1, confers enhanced tolerance to dehydration in transgenic Arabidopsis［J］. Plant Cell Reports, 2012, 31（9）：1701-1711.
［21］Zhu M, Chen G, Zhang J, et al. The abiotic stress-responsive NAC-type transcription factor SlNAC4 regulates salt and drought tolerance and stress-related genes in tomato（Solanum lycopersicum）［J］. Plant Cell Reports, 2014, 33（11）：1851-1863.
［22］ Shah ST. 陆地棉中NAC家族与叶片衰老和胁迫应答相关功能的遗传学分析［D］. 北京：中国农业科学院, 2013.

[1]	LIU Kui, LI Xing-fen, YANG Pei-xin, ZHONG Zhao-chen, CAO Yi-bo, ZHANG Ling-yun. Functional Study and Validation of Transcriptional Coactivator PwMBF1c in Picea wilsonii [J]. Biotechnology Bulletin, 2023, 39(5): 205-216.
[2]	WEI Ming WANG Xin-yu WU Guo-qiang ZHAO Meng. The Role of NAD-dependent Deacetylase SRT in Plant Epigenetic Inheritance Regulation [J]. Biotechnology Bulletin, 2023, 39(4): 59-70.
[3]	YAN Xiong-ying, WANG Zhen, WANG Xia, YANG Shi-hui. Microbial Sulfur Metabolism and Stress Resistance [J]. Biotechnology Bulletin, 2023, 39(11): 150-167.
[4]	ZHANG Hong-hong, FANG Xiao-feng. Advances in the Regulation of Stress Sensing and Responses by Phase Separation in Plants [J]. Biotechnology Bulletin, 2023, 39(11): 44-53.
[5]	FENG Ce-ting, JIANG Lyu, LIU Xing-ying, LUO Le, PAN Hui-tang, ZHANG Qi-xiang, YU Chao. Identification of the NAC Gene Family in Rosa persica and Response Analysis Under Drought Stress [J]. Biotechnology Bulletin, 2023, 39(11): 283-296.
[6]	ZHANG Yu-juan, LI Dong-hua, GONG Hui-hui, CUI Xin-xiao, GAO Chun-hua, ZHANG Xiu-rong, YOU Jun, ZHAO Jun-sheng. Cloning and Salt-tolerance Analysis of NAC Transcription Factor SiNAC77 from Sesamum indicum L. [J]. Biotechnology Bulletin, 2023, 39(11): 308-317.
[7]	LIU Yuan-yuan, WEI Chuan-zheng, XIE Yong-bo, TONG Zong-jun, HAN Xing, GAN Bing-cheng, XIE Bao-gui, YAN Jun-jie. Characteristics of Class II Peroxidase Gene Expression During Fruiting Body Development and Stress Response in Flammulina filiformis [J]. Biotechnology Bulletin, 2023, 39(11): 340-349.
[8]	LI Jian-jian, HE Chen-jing, HUANG Xiao-ping, XIANG Tai-he. Research Progress in the Regulation of Development and Stress Response by Long Non-coding RNAs in Plants [J]. Biotechnology Bulletin, 2023, 39(1): 48-58.
[9]	WANG Nan-nan, WANG Wen-jia, ZHU Qiang. Research Progress of microRNAs in Plant Stress Responses [J]. Biotechnology Bulletin, 2022, 38(8): 1-11.
[10]	TANG Qian-qian, LIN Chu-yu, TAO Zeng. Research Progress in Histone Demethylase in Plant [J]. Biotechnology Bulletin, 2022, 38(7): 13-22.
[11]	GU Pan, QI Xue-ying, LI Li, ZHANG Xi, SHAN Xiao-yi. Endocytosis of AtRGS1 Involved in the Regulation of G-protein-mediated Arabidopsis Development and Stress Responses [J]. Biotechnology Bulletin, 2022, 38(6): 34-42.
[12]	SUN Man-luan, GE Sai, BU Jia, ZHU Zhuang-yan. Regulation Mechanism of Ribonucleases in Escherichia coli [J]. Biotechnology Bulletin, 2022, 38(3): 234-245.
[13]	XU Ji-fen, CHEN Hong-fei, WANG Na, LIU Jing. Research Advances in Hog1 MAPK Signaling Pathway in Fungi [J]. Biotechnology Bulletin, 2022, 38(11): 32-40.
[14]	YIN Guo-liang, SUN Wen-hao, PANG Xiao-yun, SUN Fei. Application of cryo-Electron Microscopy in Molecular Botany Research [J]. Biotechnology Bulletin, 2022, 38(1): 15-32.
[15]	SUN Rui-fen, ZHANG Yan-fang, NIU Su-qing, GUO Shu-chun, LI Su-ping, YU Hai-feng, NIE Hui, MOU Ying-nan. Expression Analysis and Functional Verification of the HaACO1 Gene in Sunflower [J]. Biotechnology Bulletin, 2021, 37(9): 114-124.