Biotechnology Bulletin ›› 2018, Vol. 34 ›› Issue (6): 38-47.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0909

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Application of Selection and Optimization of Promoter in Metabolic Engineering of Saccharomyces cerevisiae

WANG Ke-wen ,YIN Xue, WANG Yu ,LI Yu-hua   

  1. College of Life Science,Northeast Forestry University,Haerbin 150040
  • Received:2017-10-26 Online:2018-06-26 Published:2018-07-03

Abstract: Saccharomyces cerevisiae,the simplest eukaryote,is widely used in the life science as a model organism. So far,the majority of the natural products were extracted directly from organism,which has been gradually replaced by the approaches of synthetic biology duo to its inefficiency and consuming a lot of biological resources. Modifying its metabolic pathways and adding heterometabolic pathways of S. cerevisiae for target natural products has been an efficient approach of acquiring resources. The direct synthesis of target metabolites has become the research hot spot in synthetic biology and metabolic engineering of S. cerevisiae through optimizing and transforming the exogenous gene promoter,regulating the expression level of the exogenous gene in the host,and coordinating the metabolic pathways of hosts. Here we had a detailed introduction on the structures and types of promoters as well optimizing methods of expression in the process of synthetizing natural products from the constructed S. cerevisiae,which may provide a reference for relevant researchers to use S. cerevisiae as chassis cells in synthetic biology.

Key words: Saccharomyces cerevisiae, promoter, synthetic biology, metabolic engineering