Screening and Identification of a Salt-tolerant Growth-promoting Bacterium and Its Effect on the Growth of Maize Seedlings

doi:10.13560/j.cnki.biotech.bull.1985.2019-0145

Abstract

Abstract: A strain of salt-tolerant bacteria was screened and isolated from maize rhizosphere using 10% salt concentration LB medium. The strain was identified by morphological characteristics and 16S rDNA sequence,and its growth-promoting characteristics were analyzed. The effect of the strain on maize seedling growth under salt stress was studied by pot experiment. The results showed that the strain was Kocuria rhizophila Y1 with phosphorus solubility. Under normal conditions,when K. rhizophila Y1 was colonized in the rhizosphere of maize seedlings,the plant height and root length increased by 44.96% and 49.45%,the total chlorophyll content increased by 74.44%,respectively,while the MDA content decreased by 0.65 times. Under salt stress,plant height and root length increased by 1.2 and 1.17 times,total chlorophyll content increased by 56.24%,CAT activity increased by 0.9 times,respectively,while MDA content decreased by 0.34 times. Therefore,K. rhizophila Y1 can be used as a biological fertilizer in saline soil to alleviate the inhibition of salt to the growth of maize seedlings and subsequently to promote the growth of maize seedlings.

Key words: rhizosphere growth promoting bacteria, phosphorus solubility, growth promoting, salinization

SUN Pei, WANG Gang, ZHANG Ya-nan, LI Qian, JI Jing, YANG Dan, YUAN Dong, , WANG Chang, WANG Yu-rong, WANG Ping. Screening and Identification of a Salt-tolerant Growth-promoting Bacterium and Its Effect on the Growth of Maize Seedlings[J]. Biotechnology Bulletin, 2019, 35(8): 27-33.

References

[1] Ashraf M, Foolad MA.Improving plant abiotic-stress resistance by exogenous application of osmoprotectants glycine, betaine and proline[J]. Environ Exp Bot, 2007, 59:206-216.
[2] Munns R, Tester M.Mechanisms of salinity tolerance[J]. Annu Rev Plant Biol, 2008, 59:651-681.
[3] Agmi RA.Alleviating the adverse effects of NaCl stress in maize seedings by pretreating seeds with salicylic acid and 24-epibrassinolide[J]. South Afr J Bot, 2013, 88:171-177.
[4] Khodarahmpour Z, Ifar M, Motamedi M, et al.Effects of NaCl salinity on maize(Zea mays L.)at germination and early seedling stage[J]. Afr J Biotechnol, 2012, 11:298-304.
[5] Jiang H, Qi P, Wang T, et al.Role of halotolerant phosphate-solubilising bacteria on growth promotion of peanut(Arachis hypogaea)under saline soil[J]. Ann Appl Biol, 2019, 174:20-30.
[6] Lucio V, Liliana I, Adriana F.Growth promotion of rapeseed(Bra-;ssica napus)associated with the inoculation of phosphate solubili-zing bacteria[J]. Applied Soil Ecology, 2018, 132:1-10.
[7] Aslam F, Ali B.Halotolerant bacterial diversity associated with Suaeda fruticosa(L.)forssk. improved growth of maize under Salinity Stress[J]. Agronomy-Basel, 2018, 8(8), 131.
[8] Lavecchia A, Curci M, Jangid K, et al.Microbial 16S gene-based composition of a sorghum cropped rhizosphere soil under different fertilization managements[J]. Biology and Fertility of Soils, 2015, 51(6):661-672.
[9] 马骢毓. 民勤退耕区次生草地土壤微生物多样性研究及优势植物根际促生菌资源筛选[D]. 兰州:甘肃农业大学, 2017.
[10] 东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京:科学出版社, 2001.
[11] 布坎南RE, 吉本斯NE. 伯杰细菌鉴定手册[M]. 第8版. 北京:科学出版社, 1984.
[12] 夏北成. 分子生物学方法在微生物生态学中的应用[J]. 中山大学学报:自然科学版, 1998, 37(2):97-101.
[13] Wang M, Wang G, Ji J, et a1. The effect of pds gene silencing on chloroplast pigment composition, thylakoid membrane structure and photosynthesis efficiency in tobacco plants[J]. Plant Sci, 2009, 177(3):222-226.
[14] 徐朗莱, 叶茂炳. 过氧化物酶活力连续记录测定法[J]. 南京农业大学学报, 1989, 12(3):82-83.
[15] Giannopolitis CN, Ries SK.Purification and quantitative relationship with water-soluble protein in seedling[J]. Plant Physiology, 1977, 59:315-318.
[16] Aebi H.Catalase in vitro[J]. Methods Enzymol, 1984, 105(105):121-126.
[17] Saranga Y, Kim YH, Janick J.Changes in tolerance to partial desiccation and in metabolite content of celery somatic embryos induced by reduced osmotic potential[J]. Journal of the American Society for Horticulturalence, 1992, 117(2):342-345.
[18] Shrivastava P, Kumar R.Soil salinity:A serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation[J]. Saudi J Biol Sci, 2015, 22(2):123-131.
[19] Abhilash PC, Srivastava S, Singh N.Comparative bioremediation potential of four rhizospheric microbial species against lindane[J]. Chemosphere, 2011, 82(1):56-63.
[20] Fallah MJ, Amir M, Nahid A, et al.Draft Genome sequence of Kocuria rhizophila RF, a radiation-resistant soil isolate[J]. Genome Announcements, 2016, 4(2):15-16.
[21] 陈鹏, 黄运红, 李非, 等. 炭样小单孢菌JXNU-1产抗生素JX对嗜根考克氏菌肽聚糖合成的影响及其机理[J]. 基因组学与应用生物学, 2016, 35(3):603-609.
[22] 李国辉, 李亿红, 钱冉, 等. 梨III型聚酮合成酶家族的比较基因组学研究及表达模式分析[J]. 植物生理学报, 2018, 54(6):1005-1017.
[23] Tiwari S, Singh P, Tiwari R, et al.Salt-tolerant rhizobacteria-mediated induced tolerance in wheat(Triticum aestivum)and chemical diversity in rhizosphere enhance plant growth[J]. Biol Fertility Soils, 2011, 47, 907-916.
[24] 赵清. 拟南芥chyB和DREB2A基因对转基因烟草抗逆的研究[D]. 天津:天津大学, 2013.
[25] 潘晶, 黄翠华, 罗君, 等. 盐胁迫对植物的影响及AMF提高植物耐盐性的机制[J], 地球科学进展, 2018, 33(4):361-372.
[26] 尹永强, 胡建斌, 等. 植物叶片抗氧化系统及其对逆境胁迫的响应研究进展[J]. 中国农学通报, 2007, 23(1):105-110.