Abstract: In order to obtain plant growth promoting rhizobacteria（PGPR）of wild soybean and clarify its growth-promoting characteristics,a selective culture method was used to isolate and screen nitrogen-fixing bacteria and phosphorus-solubilizing bacteria from wild soybean nodules and rhizosphere soils growing along Puhe River in Shenbei New District,Shenyang. Morphological,physiological and biochemical indicators and molecular biological identification of 16S rDNA were adapted to identify their biology,and touch-Up PCR（Rising PCR）method was employed to amplify the structural gene nifH of nitrogenase. Their phosphorus release,IAA secretion,and ACC deaminase activity were determined and analyzed for screening fine strains that then were used to study their growth-promoting effect on potted soybean. As a result,12 PGPR strains of wild soybean were obtained and identified as Bacillus,Burkholderia,Rhizobium,Janthinobacterium,Sphingomyces,Phingomonas,Mycobacterium,Pseudomonas,and Agrobacterium;2 of them dissolved organic phosphorus,11 solubilized inorganic phosphorus,3 secreted IAA,and 5 presented ACC deaminase activity. Pot experiments showed that 3 strains（GD11,GD17,and GD58）resulted in the plant height increase of soybean seedlings,5 strains（GD9,GD11,GD17,GD30,and GD58）promoted root length,and 6 strains led to the increment of stem weight,leaf dry weight,and root dry weight of soybean seedlings. Soybean root nodule and rhizosphere soil contain multi-functional rhizosphere growth-promoting bacteria,thus it has potential application value in agricultural production.

Key words: wild soybean, plant growth promoting rhizobacteria, nifH gene, phosphate-solubilizing