Isolation and Identification of Penicillium oxalicum SSCL-5 Degrading Cypermethrin and Its Biodegradation

doi:10.13560/j.cnki.biotech.bull.1985.2019-1072

Abstract

Abstract: To find a solution of removing pyrethroid pesticide residues in soil,enrichment and screening methods were used,and a microbial strain,which efficiently decomposed and utilized pyrethroid-like pesticides,was isolated from over 70 soil samples,and named as SSL-5. The strain grew normally in the mineral medium containing 1 000 mg/L cypermethrin. According to the results of morphology,ITS Sequencing analysis,the strain SSCL-5 was identified as Penicillium oxalicum,and tolerant to other several pyrethroid-like pesticides in high concentration. Degradation rate of cypermethrin（400 mg/L）by this SSL-5 in the mineral medium at 28℃ and 180 r/min and culturing 24 h was verified to be 97% with HPLC and UV spectrophotometry. Laboratory soil tests proved that cypermethrin in soil（400 mg/L）was degraded by 67.6% in 30 d while temperature in 20℃-34℃ and moisture content in 40%-60%.

Key words: fungi, cypermethrin, Penicillium oxalicum, biodegradation

CHEN Rui, QU Jia, SUN Xiao-yu, DENG Yuan, MEN Xin, ZHAO Ling-xia, SHEN Wei-rong. Isolation and Identification of Penicillium oxalicum SSCL-5 Degrading Cypermethrin and Its Biodegradation[J]. Biotechnology Bulletin, 2020, 36(6): 120-127.

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