Biological Characteristics and Antibiotic Resistance of Erysipelothrix rhusiopathiae Isolated from East China

doi:10.13560/j.cnki.biotech.bull.1985.2020-0337

Abstract

Abstract: The aim of this study is to investigate the biological characteristics of Erysipelothrix rhusiopathiae originated from pig farms in East China and its resistance to common clinical antibiotics. A total of 266 samples of pigs suspected to be infected by E. rhusiopathiae in pig farms of East China during 2017-2018 were collected and isolated,their biochemical characteristics,serotypes,mouse pathogenicity and other biological properties of the isolates were analyzed,and their 16S rDNA sequences and drug sensitivity were determined. As results,20 E. rhusiopathiae strains were isolated and identified,all of them were classified into serotype 1a in serum type,and an led to 100% mortality in challenged mice,i.e.,they had the biochemical characteristics of E. rhusiopathiae. The homology of 16S rDNA sequence with domestic reference strains and other clinical strains of E. rhusiopathiae was > 99%. Using challenge with lethal dose of 5 field strains of E. rhusiopathiae by intraperitoneal inoculation,mice immunized with commercial attenuated vaccine of E. rhusiopathiae were 100% protected from the challenge. All isolated strains were sensitive to penicillin,ampicillin,erythromycin,tilmicosin,ceftriaxone,ceftiofur and florfenicol. However,there were multi-drug resistances to lincomamides,quinolones,aminoglycosides,tetracyclines,sulfonamides,and polypeptides at varied levels. In conclusion,this study clarified the biological characteristics and antibiotic resistance of E. rhusiopathiae in East China,and the current commercial attenuated vaccine of E. rhusiopathiae can completely protect the pigs from challenges with the tested strains,which provides data for the effective prevention and control of swine erysipelas in clinical practice.

Key words: Erysipelothrix rhusiopathiae, isolation, identification, biological characteristics, antibiotic resistance

GONG Zheng, ZHANG Jun-jie, ZHONG Deng-ke. Biological Characteristics and Antibiotic Resistance of Erysipelothrix rhusiopathiae Isolated from East China[J]. Biotechnology Bulletin, 2020, 36(6): 174-182.

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