Analysis of Genetic Diversity and Genetic Structure in 7 Local Goat Breeds

doi:10.13560/j.cnki.biotech.bull.1985.2019-1069

Abstract

Abstract: Total 15 SSR sites were selected to examine the genetic diversity and genetic structure of 7 local goat breeds,which provides the basis of protection and utilization of the local goat breeds. Blood samples of Yanshan cashmere goats,Liaoning cashmere goats,Chengde polled goats,Jining gray goats,Taihang goats,Wu’an goats and ear tissues of Inner Mongolia cashmere goats were collected to investigate genetic diversity and genetic structure with microsatellite marker method. The average number of effective alleles,average expected heterozygosity and average polymorphism information content of the seven goat populations were 4.235 8,0.718 8 and 0.706 8,respectively,indicating that these seven goat breeds had high genetic diversity. The Fit（population inbreeding number）was 0.0883,average Fst（coefficient of gene differentiation among populations）was 0.544 2 and average Nm（gene flow）was 0.209 4. The genetic distance between Wu’an goat and Chengde polled goat was the closest,and that between Taihang goat and Yanshan cashmere goat was the longest. UPGMA analysis showed that Yanshan cashmere goats and Liaoning cashmere goats clustered in one,as well as Jining gray goats,Chengde polled goats,Wu’an goats,Inner Mongolia cashmere goats and Taihang goats clustered in one. In sum,the genetic diversity is abundant in seven local goats with high degree of genetic differentiation. There is a little gene exchange and the influence of inbreeding is small. The results indicate the utilization value and potential are high in seven local goat breeds.

Key words: local goat breeds, microsatellite marker, genetic diversity, genetic distance

ZHANG Le-chao, LIU Yue-qin, DUAN Chun-hui, ZHANG Ying-jie, WANG Yong, GUO Yun-xia. Analysis of Genetic Diversity and Genetic Structure in 7 Local Goat Breeds[J]. Biotechnology Bulletin, 2020, 36(6): 183-190.

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