[1] 赵文婷, 魏建和, 刘晓东, 等. 植物瞬时表达技术的主要方法与应用进展[J]. 生物技术通讯, 2013, 24(2):294-300. [2] Chen Q .Transgenic plants and beyond volume 86 || recombinant therapeutic molecules produced in plants[J]. Advances in Botanical Research, 2018, 86:207-244. [3] 霍琳, 及晓宇, 王玉成. 农杆菌介导的烟草瞬时表达试验条件优化[J]. 分子植物育种, 2016, 14(1):80-85. [4] WroblewskiT, Tomczak A, Michelmore R. Optimization of Agrobacterium-mediated transient assays of gene expression in lettuce, tomato and Arabidopsis[J]. Plant Biotechnology Journal, 2005, 3(2):259-273. [5] Sheludko Y.Agrobacterium-mediated transient expression as an approach to production of recombinant proteins in plants[J]. Recent Patents on Biotechnology, 2008, 2(3):198-208. [6] 韩岚, 张金然, 吴娜, 等. 外源基因在转基因植物中高效表达的研究进展[J]. 北京农业, 2015, 640(35):203-204. [7] 王利军, 范三红, 郭蔼光. 拟南芥atslA基因启动子的克隆和功能分析[J]. 西北植物学报, 2004, 24(10):1856-1860. [8] Diamos AG, Rosenthal SH, Mason HS.5’ and 3’ untranslated regions strongly enhance performance of geminiviral replicons in Nicotianabenthamianaleaves[J]. Front Plant Sci, 2016, 7:200. [9] Rosenthal SH, Diamos AG, Mason HS.An intronless form of the tobacco extensin gene terminator strongly enhances transient gene expression in plant leaves[J]. Plant Molecular Biology, 2018, 96(4-5):429-443. [10] Beyene G, Buenrostro-Nava MT, Damaj MB, et al.Unprecedented enhancement of transient gene expression from minimal cassettes using a double terminator[J]. Plant Cell Reports, 2011, 30(1):13-25. [11] 黄慧珍, 陈士云, 吉万全, 等. 核基质附着区与转基因表达[J]. 中国生物工程杂志, 2004(9):2-6. [12] Diamos AG, Mason HS, Chimeric 3’ flanking regions strongly enhance gene expression in plants[J]. Plant Biotechnol J, 2018, 16:1971-1982. [13] Mardanova ES, Blokhina EA, Tsybalova LM, et al.Efficient transient expression of recombinant proteins in plants by the novel pEffvector based on the genome of potato virusX[J]. Frontiers in Plant Science, 2017, 8:247. [14] Chiba M, Reed JC, ProkhnevskyAI, et al. Diverse suppressors of RNA silencing enhance agroinfection by a viral replicon[J]. Virology, 2006, 346(1):7-14. [15] Marillonnet S, Thoeringer C, Kandzia R, et al.Systemic Agrobac-terium tumefaciens-mediated transfection of viral replicons for efficient transient expression in plants[J]. Nature Biotechnology, 2005, 23(6):718-723. [16] 李晓君, 王绍梅, 谢艳兰, 等. 农杆菌渗透法转化烟草条件的优化[J]. 江苏农业科学, 2014, 42(9):45-47. [17] 孙春莲, 王洪洋, 田振东. 农杆菌介导的pCB302-3载体在本氏烟中瞬时表达条件优化[J]. 华中农业大学学报, 2015, 34(3):8-12. [18] 孙家利, 闫晓红, 王力军, 等. 烟草rbcS启动子的克隆与活性鉴定[J]. 中国烟草学报, 2010(3):86-91. [19] Agarwal P, Garg V, Gautam T, et al.A study on the influence of different promoter and 5'UTR(URM)cassettes from Arabidopsis thalianaon the expression level of the reporter gene β glucuroni-dasein tobacco and cotton[J]. Transgenic Research, 2014, 23(2):351-363. [20] Halweg C, Thompson WF, Spiker S.The rb7 matrix attachment region increases the likelihood and magnitude of transgene expression in tobacco cells:a flow cytometricstudy[J]. Plant Cell, 2005, 17(2):418-429. [21] Yamamoto T, Hoshikawa K, Ezura K, et al.Improvement of the transient expression system for production of recombinant proteins in plants[J]. Scientific Reports, 2018, 8(1):4755. [22] Marsian J, Fox H, Bahar MW, et al.Plant-made polio type 3 stabilized VLPs—a candidate synthetic polio vaccine[J]. Nature Communications, 2017, 8(1):245. [23] Lou Y, Zhou HS, Han Y, et al.Positive regulation of AMS by TDF1 and the formation of a TDF1-AMS complex are required for another development in Arabidopsis thaliana[J]. New Phytologist, 2018, 217:378-391. [24] Gallie DR, Virginia W.Identification of the motifs within the tobacco mosaic virus 5'-leader responsible for enhancing translation[J]. Nucleic Acids Research, 1992, 20(17):4631-4638. [25] Filchakova O, McIntosh JM. Functional expression of human α9* nicotinic acetylcholine receptors in X. laevis oocytes is dependent on the α9 Subunit 5' UTR[J]. PLoS One, 2013, 8(5):e64655. [26] Mlynárová L, Hricová A, LoonenA, et al. The presence of a chromatin boundary appears to shield a transgene in tobacco from RNA silencing[J]. The Plant Cell, 2003, 15(9):2203-2217. [27] Ji L, Xu R, Lu L, et al.TM6, a novel nuclear matrix attachment region, enhances its flanking gene expression through influencing their chromatin structure[J]. Molecules and Cells, 2013, 36(2):127-137. [28] Phan HA, Iacuone S, Parish LR W.The MYB80 transcription factor is required for pollen development and the regulation of tapetal programmed cell death in Arabidopsis thaliana[J]. The Plant Cell, 2011, 23(6):2209-2224. |