Effects of Livestock Manure and Straw Returning to Field on Microbial Community Structure Around Maize Rhizosphere

doi:10.13560/j.cnki.biotech.bull.1985.2020-0913

Abstract

Abstract: Livestock manure and straw as organic matter may increase the abundance and diversity of soil microorganisms,improve soil grain structure and thus increase crop yield. In this study,microorganisms around maize rhizosphere were used as the research object to explore the effects of livestock manure and straw returning to field on the microbial structure. The Illumina Miseq technology was used for high-throughput sequencing of the V4-V5 variable region of 16S rDNA gene from the maize rhizobacteria of two treatments（LM group：livestock manure;SR group：straw returning to field）. The results showed that：1）there was no significant difference in Shannon index,Simpson index and Chao1 index between SR group and LM group（P >0.05）,both had high flora abundance;2）the main phyla composition was similar at the phylum level,the relative abundance of Firmicutes and Chloroflexi in SR group was higher than that in LM group（P < 0.01）,and the relative abundance of Proteobacteria in LM group was higher than that in SR group（P < 0.01）;3）the main genus composition was similar at the genus level,the relative abundance of Bacillus spp. in SR group was higher than that in LM group（P < 0.01）;4）the functional prediction of rhizosphere microbial community mainly concentrated in the metabolic pathways of substances such as carbon,amino acids,coenzymes and vitamins,terpenoids and polyketides,etc. The above results revealed that the microbial community structure of maize rhizosphere was similar under the application of livestock manure and straw returning to field,only the proportion of some bacteria was different,but the difference of effect on maize yield was not obvious（P > 0.05）,indicating that continuous straw returning to field had the same effect as livestock manure applied.

Key words: straw returning to field, livestock manure, rhizosphere microorganism, 16S rDNA, maize

XU Lai-peng, WAN Xian-hua, SUN Xiang-li, CAO Yan-fang, LI Hui, TIAN Ya-dong, LIU Xiao-jun, KANG Xiang-tao, WANG Yan-bin. Effects of Livestock Manure and Straw Returning to Field on Microbial Community Structure Around Maize Rhizosphere[J]. Biotechnology Bulletin, 2020, 36(9): 137-146.

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