Identification and Expression of NAC Transcription Factor FtNAC17 in Tartary Buckwheat

doi:10.13560/j.cnki.biotech.bull.1985.2020-0556

Abstract

Abstract:

Plant specific NAC transcription factors are involved in plant growth and development and stress response. To study the response of NAC transcription factors in tartary buckwheat to abiotic stress would provide theoretical basis for elucidating gene function. A NAC family’s gene from tartary buckwheat was cloned and then analyzed by bioinformatics. The response of the gene to drought,low temperature,salt,methyl jasmonate,abscisic acid and gibberellin was detected by fluorescence quantitative PCR. The gene encodes 272 amino acids,which is named FtNAC17 and GenBank accession number is MT641452. The analysis of gene structure showed that FtNAC17 gene was composed of two exons and one intron. Multiple alignment of amino acid sequences and phylogenetic analysis revealed that FtNAC17 protein was the closest relative to ANAC002 in Arabidopsis thaliana,belonging to the same subgroup of NAC transcription factor family. FtNAC17 presented varied responses to drought,low temperature and salt abiotic stress.

Key words: tartary buckwheat, FtNAC17, cloning, expression, abiotic stresses

RONG Yu-ping, TANG Bin, LI Peng, ZHANG Jie-qiong, CHEN Qing-fu, ZHU Li-wei, DENG Jiao, HUANG Juan. Identification and Expression of NAC Transcription Factor FtNAC17 in Tartary Buckwheat[J]. Biotechnology Bulletin, 2021, 37(1): 174-181.

Figures/Tables 9

References 30

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位置	可能性/%
微体	64
细胞质	45
线粒体	1

位置	可能性/%
微体	64
细胞质	45
线粒体	1