Heterologous Expression of Quinone Oxidoreductase and Its Role in the Decolorization of Azo Dyes

doi:10.13560/j.cnki.biotech.bull.1985.2020-1454

Abstract

Abstract:

In order to study the potential of NAD（P）H:quinone oxidoreductase in dye decolorization,heterologous expressed human source NAD（P）H:quinone oxidoreductase（hNQO1）in Escherichia coli was used to decolorize the azo dye in the presence of mediator at proper concentration. Six small molecular mediators（1,4-naphquinone,sodium anthraquinone-2-sulfonate,2-methlcyclohexa-2,5-diene-1,4-dione,1,4-dihydroxyanthracene-9,10-dion,anthraquinone,and menadione）were investigated for hNQO1. The results showed that hNQO1 whole cell decolorized Congo red very well with 1,4-dihydroxyanthracene-9,10-dion as the small molecular mediator. The hNQO1 whole cells could be reused for three times and the decolorization rate（6 h）was 94.0%,79.7%,and 20.3% respectively. Compared with Congo red,the decolorization rate of hNQO1 whole cells on amaranth and reactive black 5（6 h）was only 18.8% and 16.4%,respectively. In conclusion,the recombinant hNQO1 presents great potential for dye wastewater treatment.

Key words: quinone oxidoreductase, dye decolorization, mediator

LIU Qian-qian, TANG Zi-jing, LI Tian-zhen, LI Bao-ku, ZHU Lei-lei. Heterologous Expression of Quinone Oxidoreductase and Its Role in the Decolorization of Azo Dyes[J]. Biotechnology Bulletin, 2021, 37(10): 128-136.

Figures/Tables 8

Fig.1 SDS-PAGE of hNQO1 heterologously expressed A:M:protein marker;1:induced hNQO1;2: supernatant after crushing. B: M:protein marker;1: purified hNQO1

Fig. 2 Influence of thermal stability on enzyme activity

Table 1 Dye names and their maximum absorption wavelengths

名称Name	分子质量Molecular mass/kD	吸收波长Absorption wavelength/nm
刚果红 Congo red	696.7	434
苋菜红 Amaranth	604.47	520
活性黑5 Reactive black 5	991.82	596

Table 2 Names and structures of small molecular mediators

名称Name	化学结构Chemical structural formula	分子质量Molecular mass/kD
1,4-二羟基蒽醌 1,4-Dihydroxyant- hracene-9,10-dion		240.2109
1,4-萘醌 1,4-Naphthoqu- inone		158.15
甲萘醌 Menadione		172.18
蒽醌 Anthraquinone		208.2121
甲基对苯醌 2-Methlcyclohexa-2,5-diene-1,4-dione		122.1213
蒽醌-2-磺酸钠 Sodium anthraqu-inone-2-Sulfonate		310.2571

Fig. 3 Influence of different medium small molecules on the decolorization of hNQO1 Congo red

Fig. 4 Effects of 1,4-Dihydroxyanthracene-910-dion at different concentrations on the decolorization of hNQO1 Congo red

Fig. 5 Decolorization of Congo red dye by reusing whole cells of hNQO1

Fig. 6 Decolorization of two azo dyes such as amaranth and reactive black 5 by whole cells of hNQO1

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