Bioremediation of Cr（VI）-contaminated Farmland Soil by Microbacterium sp. BD6

doi:10.13560/j.cnki.biotech.bull.1985.2021-0194

Abstract

Abstract:

The purpose of this paper is to optimize the conditions and investigate the effects in bioremediation of Cr（VI）-contaminated soil by Cr（VI）-reducing bacteria,and to provide reference in practical remediation of Cr（VI）-contaminated soil. The single factor experiments,the pot experiments,high-throughput sequencing and qPCR methods were used to study the optimal conditions for the bioremediation of Cr（VI）-contaminated soil by Microbacterium sp. BD6,the toxicity to plants before and after remediation,and the effects on soil microbial community in the soil. The experiments showed that the Cr（VI）reduction rate of 100 mg Cr（VI）/kg contaminated soil by strain BD6 was above 90% in 96 h under the conditions of soil moisture content 30% and temperature 25℃- 40℃. Strain BD6 still reduced Cr（VI）in presence of other heavy metal ions,but the reduction effects were impacted. Cr（VI）restrained the height,germination rate and other indicators of soybean plant. The toxicity of the soil remediated by strain BD6 was reduced,the growth of plants was significantly improved,and the chromium content in plants significantly decreased. The microbial abundance and diversity in Cr（VI）-contaminated soil were significantly lower than those in non-contaminated soil. The microbial abundance and diversity of Cr（VI）-contaminated soil after remediation by strain BD6 increased with the bioremediation proceeded continuously. In conclusion,strain BD6 can be used as a potential candidate strain for the remediation of Cr（VI）-contaminated soil in the future.

Key words: Microbacterium sp. BD6, Cr（VI）-contaminated soil, bioremediation, microbial community, electron donor

YANG Zong-zheng, ZHAO Xiao-yu, LIU Dan, XU Wen-shuai, WU Zhi-guo. Bioremediation of Cr（VI）-contaminated Farmland Soil by Microbacterium sp. BD6[J]. Biotechnology Bulletin, 2021, 37(10): 81-90.

Figures/Tables 6

References 26

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土壤修复情况 Soil remediation	土壤Cr（VI）含量Cr（VI） content in soil/（mg·kg^-1）	发芽率 Germination rate/%	植株高度 Hight of plants/mm	植株鲜重 Fresh weight of plants（g/plant）	植株铬含量Chromium content in plants/（mg·kg^-1）
未修复 Non-remediated	0	100.0 a	104±2.1 a	0.64±0.04 a	未检出 g
	5	100.0 a	89±0.42 b	0.51±0.02 c	0.8±0.1 f
	10	100.0 a	55±1.6 c	0.49±0.02 c	1.1±0.3 f
	20	100.0 a	30±0.9 d	0.37±0.02 d	1.6±0.1 e
	30	100.0 a	29±0.8 d	0.36±0.01 de	2.1±0.1 d
	50	100.0 a	25±1.2 e	0.33±0.02 ef	2.6±0.3 c
	80	100.0 a	15±0.3 f	0.30±0.01 f	3.8±0.1 b
	100	88.9±4.84 b	12±0.1 g	0.26±0.01g	5.0±0.4 a
已修复Remediated	100	100.0 a	87±0.9 b	0.56±0.03 b	未检出 Unolected

土壤修复情况 Soil remediation	土壤Cr（VI）含量Cr（VI） content in soil/（mg·kg^-1）	发芽率 Germination rate/%	植株高度 Hight of plants/mm	植株鲜重 Fresh weight of plants（g/plant）	植株铬含量Chromium content in plants/（mg·kg^-1）
未修复 Non-remediated	0	100.0 a	104±2.1 a	0.64±0.04 a	未检出 g
	5	100.0 a	89±0.42 b	0.51±0.02 c	0.8±0.1 f
	10	100.0 a	55±1.6 c	0.49±0.02 c	1.1±0.3 f
	20	100.0 a	30±0.9 d	0.37±0.02 d	1.6±0.1 e
	30	100.0 a	29±0.8 d	0.36±0.01 de	2.1±0.1 d
	50	100.0 a	25±1.2 e	0.33±0.02 ef	2.6±0.3 c
	80	100.0 a	15±0.3 f	0.30±0.01 f	3.8±0.1 b
	100	88.9±4.84 b	12±0.1 g	0.26±0.01g	5.0±0.4 a
已修复Remediated	100	100.0 a	87±0.9 b	0.56±0.03 b	未检出 Unolected

Sample ID	Ace	Chao1	Shannon	Simpson	Coverage
Y	2527.894±43.237 a	2500±70.335 a	9.284±0.035 a	0.991±0.001 a	0.987±0.002
Cr	1785.987±91.525 e	1811.426±77.381 de	8.336±0.368 b	0.972±0.004 ab	0.994±0.006
A-96h	1974.381±83.318 cd	1887.949±47.887 cd	5.209±0.837 e	0.796±0.071 d	0.982±0.001
G-96h	2317.543±142.074 b	2262.373±90.249 b	6.911±0.386 cd	0.921±0.009 c	0.982±0.001
A-10d	1850.619±60.012 de	1691.645±54.171 e	6.071±0.265 de	0.936±0.011 bc	0.983±0.001
G-10d	2001.833±81.225 cd	1878.924±93.194 cd	6.791±0.078 cd	0.971±0.005 ab	0.983±0.000
A-15d	2079.836±96.326 c	1963.311±68.010 c	7.228±0.368 c	0.977±0.002 ab	0.981±0.003
G-15d	1548.096±74.238 f	1482.651±72.5451 f	6.522±1.014 cd	0.965±0.003 abc	0.986±0.002

Sample ID	Ace	Chao1	Shannon	Simpson	Coverage
Y	2527.894±43.237 a	2500±70.335 a	9.284±0.035 a	0.991±0.001 a	0.987±0.002
Cr	1785.987±91.525 e	1811.426±77.381 de	8.336±0.368 b	0.972±0.004 ab	0.994±0.006
A-96h	1974.381±83.318 cd	1887.949±47.887 cd	5.209±0.837 e	0.796±0.071 d	0.982±0.001
G-96h	2317.543±142.074 b	2262.373±90.249 b	6.911±0.386 cd	0.921±0.009 c	0.982±0.001
A-10d	1850.619±60.012 de	1691.645±54.171 e	6.071±0.265 de	0.936±0.011 bc	0.983±0.001
G-10d	2001.833±81.225 cd	1878.924±93.194 cd	6.791±0.078 cd	0.971±0.005 ab	0.983±0.000
A-15d	2079.836±96.326 c	1963.311±68.010 c	7.228±0.368 c	0.977±0.002 ab	0.981±0.003
G-15d	1548.096±74.238 f	1482.651±72.5451 f	6.522±1.014 cd	0.965±0.003 abc	0.986±0.002