Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (11): 267-275.doi: 10.13560/j.cnki.biotech.bull.1985.2020-1506

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Identification and Function Study of a New Type of sRNA N155 from Aeromonas veronii

HE Jin-hua1(), MA Xiang1, TANG Yan-qiong1, WANG Dan2, LI Hong1(), LIU Zhu1   

  1. 1. School of Life Science and Pharmacy,Hainan University,Haikou 570228
    2. College of Tropical Crops,Hainan University,Haikou 570228
  • Received:2020-12-13 Online:2021-11-26 Published:2021-12-03
  • Contact: LI Hong E-mail:hejinhua0625@163.com;lihongbio@163.com

Abstract:

Aeromonas veronii is a new type of pathogen that can infect both fish and mammals including humans. and its pathogenic mechanism is not fully understood. A new sRNA N155 in wild type A. veronii C4 was identified based on the transcriptome data,and molecular biological methods such as homologous recombination were used to construct the sRNA N155 deletion strain. The results of transcriptome data and RT-qPCR analysis showed that sRNA N155 was independently transcribed. Sequence alignment analysis indicated that sRNA N155 was species-specific and only existed in Aeromonas. The results of growth curve determination and swimming motility experiments revealed that the knockout of sRNA N155 didn’t affect the growth of A.veronii,but caused the swimming motility of A. veronii reduced. The ΔsRNA N155 strain successfully constructed in this study provides a reliable material for further research on the function of sRNA N155 in A. veronii.

Key words: Aeromonas veronii, transcriptome, sRNA, swimming motility