Recent Advances on Dynamic Regulation and Imaging Techniques of RNA Polymerase II

doi:10.13560/j.cnki.biotech.bull.1985.2020-1015

Abstract

Abstract:

RNA polymerase II（RNAP II or Pol II）plays a vital role in regulating the growth and development of organisms and responding to environmental changes. RNA polymerase II assembles and locates at transcription factory,where multiple genes are recruited for the formation of co-transcription,which is a highly dynamic and coordinated process. Although the critical functions of RNA polymerase II in eukaryotic gene transcription have been widely studied,the dynamic characteristics of plant RNAP II under different physiological conditions are still poorly understood. The development of single-molecule imaging and labeling techniques in living cell will contribute to improving our new understanding of the dynamic characteristics of plant RNAP II in vivo. In this review,we mainly expound the structural and functional characteristics of RNAP II,and introduce the dynamic regulation and transcription mechanism of RNAP II. Furthermore,we put the emphasis on summarizing the recent progresses in dynamic labeling and imaging techniques of RNAP II in living cells,aiming to provide theoretical basis for further studies on the dynamic regulation of RNAP II in plant.

Key words: transcription factory, dynamic labeling technology, imaging technique of single-molecule in vivo, regulatory mechanism

QIAN Hong-ping, CHEN Bo, LIN Jin-xing, CUI Ya-ning. Recent Advances on Dynamic Regulation and Imaging Techniques of RNA Polymerase II[J]. Biotechnology Bulletin, 2021, 37(4): 293-302.

Figures/Tables 3

References 61

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参数Parameter	PALM	STORM	STED	LSFM	Bayesian nanoscopy
分辨率Resolution/nm	<30	<30	20-70	亚细胞分辨率 Subcellular resolution	50 nm
成像深度Imaging depth/μm	<10	<10	<100	通常<50	通常<50
成像速度Imaging speed	慢Slow	慢-低Slow to low	中等Medium	非常快Very fast	非常快Very fast
内源性Endogenous	无No	有Yes	有Yes	有Yes	有Yes
光毒性Phototoxicity	中到高Medium to high	中等Medium	高High	低Low	低 Low
蛇币价格Equipment price	中等Medium	中等Medium	非常昂贵Very expensive	非常昂贵Very expensive	非常昂贵Very expensive

参数Parameter	PALM	STORM	STED	LSFM	Bayesian nanoscopy
分辨率Resolution/nm	<30	<30	20-70	亚细胞分辨率 Subcellular resolution	50 nm
成像深度Imaging depth/μm	<10	<10	<100	通常<50	通常<50
成像速度Imaging speed	慢Slow	慢-低Slow to low	中等Medium	非常快Very fast	非常快Very fast
内源性Endogenous	无No	有Yes	有Yes	有Yes	有Yes
光毒性Phototoxicity	中到高Medium to high	中等Medium	高High	低Low	低 Low
蛇币价格Equipment price	中等Medium	中等Medium	非常昂贵Very expensive	非常昂贵Very expensive	非常昂贵Very expensive