The Strategy for Enhancing Foreign Proteins Expression by Signal Peptide in Bacillus subtilis

doi:10.13560/j.cnki.biotech.bull.1985.2020-1255

Abstract

Abstract:

As an important prokaryotic expression host strain,Bacillus subtilis has always been regarded as preferred model strain for foreign protein expression,featuring strong protein secretion,clear genetic background,no codon preference,rapid growth and non-pathogenicity. Signal peptide is a short peptide chain located in the N-terminal of the precursor protein,with the function of guiding and regulating the folding of the precursor protein. Meanwhile,it plays a very important role in the process of protein transfer and secretion. At present,there is no regularity to find the efficient secretion of different foreign proteins by using the signal peptide of B. subtilis. For this reason,the structural characteristics,classification,transport pathway and application of signal peptides from B. subtilis are reviewed here,aiming to provide certain reference for further screening the optimal signal peptides of foreign proteins in B. subtilis expression system.

Key words: Bacillus subtilis, signal peptide, secretion pathway, signal peptide library

MIAO Hua-biao, CAO Yan, YANG Meng-han, HUANG Zun-xi. The Strategy for Enhancing Foreign Proteins Expression by Signal Peptide in Bacillus subtilis[J]. Biotechnology Bulletin, 2021, 37(6): 259-271.

Figures/Tables 4

References 87

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外源蛋白 Foreign protein	信号肽类型 Signal peptide type	最适信号肽 Optimal signal peptide	分泌途径 Secretory pathway	胞外活性 Extracellular activity	参考文献 Reference
酰胺酶 Amidase	20个Sec+1个Tat	Pac	Sec	+1.36倍	[40]
氨肽酶 Aminopeptidase	19个Sec	YncM	Sec	+1.20倍	[41]
碱性丝氨酸蛋白酶 Alkaline serine protease	73个Sec	DacB	Sec	953.00 U/mg	[42]
角蛋白酶 Keratinase	5个Sec+4个Tat	LipA	Tat	+2.00倍	[43]
L-天冬酰胺酶 L-asparaginase	5个Sec+3个Tat	WapA	Tat	+1.72倍	[44]
β-甘露聚糖酶 β-mannanase	3个Sec+3个Tat	LipA	Tat	+1.25倍	[45]
碱性果胶酶 Alkaline polygalacturonate lyase	11个Sec+3个Tat	Bpr	Sec	313.70 U/g	[46]
果聚糖蔗糖酶 Encoding levansucrase	7个Sec+1个Tat	YncM	Sec	+2.61倍	[47]
普鲁兰酶 Pullulanase	4个Sec	SacB	Sec	2.82 U/mL	[48]
聚对苯二甲酸乙二醇酯水解酶 PETase	3个Sec+3个Tat	SP_PETase	Tat	+3.80倍	[49]
脂肪酶 Lipase	6个Sec+2个Tat	PhoD	Tat	+2.33倍	[50]
β-半乳糖苷酶 β-galactosidase	3个Sec+2个Tat	PhoD	Tat	+10.10倍	[51]
α-淀粉酶 α-Amylase	5个Sec+1个Tat	NprE	Sec	260.00 U/mL	[52]
碱性淀粉酶 Alkaline amylase	9个Sec+4个Tat	YwbN	Tat	364.5 U/mL	[53]
α-淀粉酶 α-Amylase	44个Sec+2个Tat	BglS	Sec	1393.30 U/mL	[54]
α-淀粉酶 α-Amylase	8个Sec+1个Tat	YfkN	Sec	+10.00倍	[55]
α-淀粉酶 α-Amylase	6个Sec+2个Tat	NprE	Sec	242.00 U/mL	[56]

外源蛋白 Foreign protein	信号肽类型 Signal peptide type	最适信号肽 Optimal signal peptide	分泌途径 Secretory pathway	胞外活性 Extracellular activity	参考文献 Reference
酰胺酶 Amidase	20个Sec+1个Tat	Pac	Sec	+1.36倍	[40]
氨肽酶 Aminopeptidase	19个Sec	YncM	Sec	+1.20倍	[41]
碱性丝氨酸蛋白酶 Alkaline serine protease	73个Sec	DacB	Sec	953.00 U/mg	[42]
角蛋白酶 Keratinase	5个Sec+4个Tat	LipA	Tat	+2.00倍	[43]
L-天冬酰胺酶 L-asparaginase	5个Sec+3个Tat	WapA	Tat	+1.72倍	[44]
β-甘露聚糖酶 β-mannanase	3个Sec+3个Tat	LipA	Tat	+1.25倍	[45]
碱性果胶酶 Alkaline polygalacturonate lyase	11个Sec+3个Tat	Bpr	Sec	313.70 U/g	[46]
果聚糖蔗糖酶 Encoding levansucrase	7个Sec+1个Tat	YncM	Sec	+2.61倍	[47]
普鲁兰酶 Pullulanase	4个Sec	SacB	Sec	2.82 U/mL	[48]
聚对苯二甲酸乙二醇酯水解酶 PETase	3个Sec+3个Tat	SP_PETase	Tat	+3.80倍	[49]
脂肪酶 Lipase	6个Sec+2个Tat	PhoD	Tat	+2.33倍	[50]
β-半乳糖苷酶 β-galactosidase	3个Sec+2个Tat	PhoD	Tat	+10.10倍	[51]
α-淀粉酶 α-Amylase	5个Sec+1个Tat	NprE	Sec	260.00 U/mL	[52]
碱性淀粉酶 Alkaline amylase	9个Sec+4个Tat	YwbN	Tat	364.5 U/mL	[53]
α-淀粉酶 α-Amylase	44个Sec+2个Tat	BglS	Sec	1393.30 U/mL	[54]
α-淀粉酶 α-Amylase	8个Sec+1个Tat	YfkN	Sec	+10.00倍	[55]
α-淀粉酶 α-Amylase	6个Sec+2个Tat	NprE	Sec	242.00 U/mL	[56]

外源蛋白 Foreign protein	信号肽 Signal peptide	分泌途径 Secretory pathway	信号肽突变点 Signal peptide mutation site	胞外活性 Extracellular activity	参考文献 Reference
脂肪酶 Lipase	PhoD	Tat	N3K、N3K/L4K、D25R、D25R/F29R	+1.12-1.31倍	[50]
碱性淀粉酶 Alkaline amylase	YwbN	Tat	E4K/E9K、I16N/ L17S/W19S	+1.08-1.58倍	[53]
α-淀粉酶 α-Amylase	YfkN	Sec	I3G/Q4R	+13.00倍	[55]
地衣聚糖酶 Lichenase	H1	-	VNIAFMLF/RKIAGMAT	+4.60倍	[57]
角质酶 Cutinase	AmyE	Sec	F2D、F2E、F6W、K4L	+2-3倍	[58]
β-半乳糖苷酶 β-galactosidase	AmyX	Tat	N4I/ Y8F/N17I	+2.50倍	[59]

外源蛋白 Foreign protein	信号肽 Signal peptide	分泌途径 Secretory pathway	信号肽突变点 Signal peptide mutation site	胞外活性 Extracellular activity	参考文献 Reference
脂肪酶 Lipase	PhoD	Tat	N3K、N3K/L4K、D25R、D25R/F29R	+1.12-1.31倍	[50]
碱性淀粉酶 Alkaline amylase	YwbN	Tat	E4K/E9K、I16N/ L17S/W19S	+1.08-1.58倍	[53]
α-淀粉酶 α-Amylase	YfkN	Sec	I3G/Q4R	+13.00倍	[55]
地衣聚糖酶 Lichenase	H1	-	VNIAFMLF/RKIAGMAT	+4.60倍	[57]
角质酶 Cutinase	AmyE	Sec	F2D、F2E、F6W、K4L	+2-3倍	[58]
β-半乳糖苷酶 β-galactosidase	AmyX	Tat	N4I/ Y8F/N17I	+2.50倍	[59]

外源蛋白 Foreign protein	信号肽类型 Signal peptide type	分泌途径 Secretory pathway	最适信号肽 Optimal signal peptide	胞外活性 Extracellular activity	参考文献Reference
角质酶 Cutinase	173个Sec	Sec	Epr	4.67 U/mL	[12]
细胞质酯酶 Cytoplasmatic esterase	173个Sec	Sec	YwmC	1.50 U/mL	[12]
α-麦芽淀粉酶 Maltogenic α-amylase	173个Sec	Sec	YvcE	250.70 U/mL	[60]
蛋白酶 Protease	173个Sec	Sec	YbdN	+7.00倍	[61]
纳豆植酸酶 Natto phytase	173个Sec	Sec	Pbp	+2.00倍	[62]
α-淀粉酶 α-Amylase	173个Sec	Sec	YomL	113.25 U/mL	[63]
α-淀粉酶 α-Amylase	173个Sec	Sec	YojL	+3.5倍	[64]
α-淀粉酶 α-Amylase	173个Sec	Sec	PeI	+1.68倍	[65]
木聚糖酶 Xylanase	114个Sec+24个Tat	Sec	PhoB	327.20 U/mL	[66]