Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (9): 125-131.doi: 10.13560/j.cnki.biotech.bull.1985.2020-1572

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Cloning,Expression and Bioinformatics Analyses of CpBURP from Commelina purpurea

PENG Guo-ying1(), LU Shan1, HUANG Chao2, YANG Kun1, WAN Wei1, HUANG Chang-gan1()   

  1. 1. Jiangxi Agricultural University/Nanchang Key Laboratory of Chemical Utilization of Plant Resources,Nanchang 330045
    2. Jiangxi Agricultural University Alumni Office,Nanchang 330045
  • Received:2020-12-27 Online:2021-09-26 Published:2021-10-25
  • Contact: HUANG Chang-gan E-mail:1114755190@qq.com;windhcg@163.com

Abstract:

BURP protein plays an important role in the growth of plant and resistance to abiotic stress. Cloning a CpBURP and analyzing its role in Commelina purpurea may lay a foundation for further deeply studying the gene BURP in C. purpurea. Screening was performed based on the C. purpurea transcriptome database and a CpBURP was cloned,and then its bioinformatics and expressions were analyzed. The result showed the CpBURP of 1 383 bp encoded a protein with 460 amino acids. The hydrophilic CpBURP protein contained a BURP conserved domain and 50 phosphorylation sites,but no transmembrane structure and signal peptide region. It is predicted that CpBURP protein acted on the cytoplasm and peroxisomes. Phylogenetic tree analysis revealed that CpBURP protein and 13 plant BURP proteins were grouped into 6 subcategories,the CpBURP protein had the closest affinity to the BURP protein in Brassica napus. The results of qRT-PCR demonstrated that the expression of CpBURP in the root was higher than that in the stem and leaf. Under the Cu2+ stress,the expression of CpBURP in the roots was significantly higher than that in the control group. CpBURP may play an important role in the response of C. purpurea to Cu2+ stress.

Key words: Commelina purpurea, CpBURP, gene cloning, bioinformatics, expression analysis