Research Progress in the Immobilization of Key Enzymes for Pesticides Residue Detection

doi:10.13560/j.cnki.biotech.bull.1985.2021-0080

Abstract

Abstract:

In order to detect pesticide residues in agro-products and foods to ensure consumers’ food safety,some fast detection techniques need to be developed urgently. Enzyme inhibition method is one of current main research directions in the rapid detection techniques of pesticide residues. While the immobilized enzyme based on the principle of enzyme inhibition method is an important step in the research of detecting pesticide residue. Applying physical or chemical methods to efficiently fix enzyme on the carrier without losing catalytic activity is the key step to develop all kinds of sensor based on enzyme inhibition method to detect pesticide residues. In this paper,the immobilized enzyme methods,carriers and the practical applications of immobilized enzyme products in pesticide residue detection are reviewed,so as to provide some experiences and references for the applications of immobilized enzyme in pesticide residue detection.

Key words: enzyme inhibition method, pesticide residues, immobilized enzyme

ZHU Yong-an, WANG Miao, CAO Jing, YU He, CAO Zhen, JIN Mao-jun, WANG Jing, SHE Yong-xin. Research Progress in the Immobilization of Key Enzymes for Pesticides Residue Detection[J]. Biotechnology Bulletin, 2022, 38(1): 258-268.

Figures/Tables 5

References 63

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MOFs名称 MOFs name	固定化酶 Immobilized enzyme	检测农药 Detection of pesticide	线性范围 Linear range	检出限 LOD	参考文献 Reference
Pt@UiO66-NH₂	AChE	马拉硫磷Malathion	1×10^-14 -1×10^-9mol/L	4.9 × 10^-15 mol/L	[44]
MXene/AuNPs/MnO₂/Mn₃O₄/GCE	AChE	甲胺磷Methamidophos	1×10^-12 -1×10^-6mol/L	1.34 × 10^-13 mol/L	[45]
MOF-Basolite Z1200/μE	AChE	毒死蜱Chlorpyrifos	10-100 ng/L	6 ng/L	[46]
UiO-66-NH₂	OPH	甲基对硫磷Parathion-methyl	10-10⁶ng/mL	10 ng/mL	[47]
Ce-UiO-66/MWCNTs/GCE	AChE	对氧磷Paraoxon	0.01-150 nmol/L	0.004 nmol/L	[48]
Fe₃O₄NPs@ZIF-8	AChE	二嗪磷Dizinon	0.5-500 nmol/L	0.2 nmol/L	[49]
MOF-Pt	AChE	毒死蜱Chlorpyrifos	0.5 ng/mL-1.0 μg/mL	0.21 ng/mL	[50]
LDH@ZIF-8	AChE	二嗪磷Dizinon	0.5-300 nmol/L	0.22 nmol/L	[51]
Cu-Hemin MOFs/NECF	AChE	敌百虫Dipterex	0.25-20 ng/mL	0.082 ng/mL	[52]

MOFs名称 MOFs name	固定化酶 Immobilized enzyme	检测农药 Detection of pesticide	线性范围 Linear range	检出限 LOD	参考文献 Reference
Pt@UiO66-NH₂	AChE	马拉硫磷Malathion	1×10^-14 -1×10^-9mol/L	4.9 × 10^-15 mol/L	[44]
MXene/AuNPs/MnO₂/Mn₃O₄/GCE	AChE	甲胺磷Methamidophos	1×10^-12 -1×10^-6mol/L	1.34 × 10^-13 mol/L	[45]
MOF-Basolite Z1200/μE	AChE	毒死蜱Chlorpyrifos	10-100 ng/L	6 ng/L	[46]
UiO-66-NH₂	OPH	甲基对硫磷Parathion-methyl	10-10⁶ng/mL	10 ng/mL	[47]
Ce-UiO-66/MWCNTs/GCE	AChE	对氧磷Paraoxon	0.01-150 nmol/L	0.004 nmol/L	[48]
Fe₃O₄NPs@ZIF-8	AChE	二嗪磷Dizinon	0.5-500 nmol/L	0.2 nmol/L	[49]
MOF-Pt	AChE	毒死蜱Chlorpyrifos	0.5 ng/mL-1.0 μg/mL	0.21 ng/mL	[50]
LDH@ZIF-8	AChE	二嗪磷Dizinon	0.5-300 nmol/L	0.22 nmol/L	[51]
Cu-Hemin MOFs/NECF	AChE	敌百虫Dipterex	0.25-20 ng/mL	0.082 ng/mL	[52]

传感器名称 Sensor name	类型 Type	农药 Pesticide	线性范围 Linear range	检出限 LOD	参考文献 Reference
AChE/e-pGON/GCE	电化学生物传感器 Electrochemical biosensor	甲萘威 Carbaryl	0.3-6.1 ng/mL	0.15 ng/mL	[59]
AChE/Au/ssDNA- SWCNT/PANI	电化学生物传感器 Electrochemical biosensor	甲基对硫磷 Parathion-methyl 毒死蜱 Chlorpyrifos	1.0×10^-11-1.0×10^-6mol/L	1.0×10^-12mol/L	[60]
Tyr-BCs/Au	电化学发光传感器 ECL	阿特拉津 Atrazine	0.0001-0.01 μg/L 0.01-20 μg/L	0.08 ng/L	[31]
ACh/CNT/NH₂	生物传感器 Biosensor	对氧磷 Paraoxon	0.2-1 nmol/L	0.08 nmol/L	[34]
AChE/GMA/EDMA	微流控芯片 Micro-fluidic chip	对氧磷 Paraoxon	0.25-2.5 mg/mL	0.17 mg/mL	[61]
AChE-FH-A-GNPs	生物传感器 Biosensor	克百威 Carbofuran 杀线威 Oxamyl 灭多威 Methomyl 甲萘威 Carbaryl	NR	2 nmol/L 21 nmol/L 113 nmol/L 236 nmol/L	[62]
AChE/Ce-UiO-66 /MWCNTs/GCE	电化学生物传感器 Electrochemical biosensor	对氧磷 Paraoxon	0.01-150 nmol/L	0.004 nmol/L	[48]
AChE/AuPt/PDA	生物传感器 Biosensor	对氧磷 Paraoxon	0.5-1000 ng/L	0.185 ng/L	[63]
GCE/P-ABSA/DAR /AuNPs/DAR/AChE	生物传感器 Biosensor	马拉硫磷 Malathion 甲基对硫磷 Parathion-methyl	0.003-30 pmol/L 0.0038-38 pmol/L	0.0016 pmol/L 0.0022 pmol/L	[9]
AChE/PET/Ag/AgCl	微流控芯片 Micro-fluidic chip	对硫磷 Parathion	1.0×10^-7-1.0×10^-5 g/mL	3.3×10^-8 g/mL	[12]
NF/AChE-CS/SNS-NF/GCE	生物传感器 Biosensor	甲基对硫磷 Parathion-methyl 毒死蜱 Chlorpyrifos 克百威 Carbofuran	10^-12-10^-10 mol/L 10^-10-10^-8mol/L	5.0×10^-13mol/L	[16]

传感器名称 Sensor name	类型 Type	农药 Pesticide	线性范围 Linear range	检出限 LOD	参考文献 Reference
AChE/e-pGON/GCE	电化学生物传感器 Electrochemical biosensor	甲萘威 Carbaryl	0.3-6.1 ng/mL	0.15 ng/mL	[59]
AChE/Au/ssDNA- SWCNT/PANI	电化学生物传感器 Electrochemical biosensor	甲基对硫磷 Parathion-methyl 毒死蜱 Chlorpyrifos	1.0×10^-11-1.0×10^-6mol/L	1.0×10^-12mol/L	[60]
Tyr-BCs/Au	电化学发光传感器 ECL	阿特拉津 Atrazine	0.0001-0.01 μg/L 0.01-20 μg/L	0.08 ng/L	[31]
ACh/CNT/NH₂	生物传感器 Biosensor	对氧磷 Paraoxon	0.2-1 nmol/L	0.08 nmol/L	[34]
AChE/GMA/EDMA	微流控芯片 Micro-fluidic chip	对氧磷 Paraoxon	0.25-2.5 mg/mL	0.17 mg/mL	[61]
AChE-FH-A-GNPs	生物传感器 Biosensor	克百威 Carbofuran 杀线威 Oxamyl 灭多威 Methomyl 甲萘威 Carbaryl	NR	2 nmol/L 21 nmol/L 113 nmol/L 236 nmol/L	[62]
AChE/Ce-UiO-66 /MWCNTs/GCE	电化学生物传感器 Electrochemical biosensor	对氧磷 Paraoxon	0.01-150 nmol/L	0.004 nmol/L	[48]
AChE/AuPt/PDA	生物传感器 Biosensor	对氧磷 Paraoxon	0.5-1000 ng/L	0.185 ng/L	[63]
GCE/P-ABSA/DAR /AuNPs/DAR/AChE	生物传感器 Biosensor	马拉硫磷 Malathion 甲基对硫磷 Parathion-methyl	0.003-30 pmol/L 0.0038-38 pmol/L	0.0016 pmol/L 0.0022 pmol/L	[9]
AChE/PET/Ag/AgCl	微流控芯片 Micro-fluidic chip	对硫磷 Parathion	1.0×10^-7-1.0×10^-5 g/mL	3.3×10^-8 g/mL	[12]
NF/AChE-CS/SNS-NF/GCE	生物传感器 Biosensor	甲基对硫磷 Parathion-methyl 毒死蜱 Chlorpyrifos 克百威 Carbofuran	10^-12-10^-10 mol/L 10^-10-10^-8mol/L	5.0×10^-13mol/L	[16]