Abstract:

Salt stress is one of the most important abiotic stresses affecting rice production. In this study,the transcriptome data of rice under salt stress were used to analyze the expression changes of transcription factors at the transcriptome level by HTSeq and DESeq software. The main results were as follows：there were 26 identical transcription factors differentially expressed in rice at 1,3 and 6 h of salt stress,and an important module containing 14 genes was screened by protein interaction. GO analysis of important modules showed that 14 genes were mainly enriched in ATP binding,stress response and cytoplasmic membrane-bounded vesicle. KEGG analysis showed that it was mainly enriched in endoplasmic reticulum protein processing and endocytosis pathway. Meanwhile,the key heat shock transcription factor gene Os03g074500 and Os06g0553100 induced by salt stress were identified. It is speculated that these two genes may play a significant role in the process of rice salt stress by regulating the transcription and expression of HSP70 gene and affecting protein folding and assembly. This study provides candidate genes for subsequent genetic improvement of rice and provides scientific enlightenment for further understanding of salt tolerance mechanism.

Key words: rice, key transcription factors, salt stress, protein interaction, transcriptome