Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (4): 242-252.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0964

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Screening of Endophytic Bacteria from Rehmannia glutinosa at Different Stages and Analysis of Their Growth-promoting Characteristics

WANG Chun-yan1,2(), LA Gui-xiao3, SU Xiu-hong1,2(), LI Meng1,2, DONG Cheng-ming1,2   

  1. 1. Henan University of Chinese Medicine,Zhengzhou 450046
    2. Co-construction of Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan & Education Ministry of P.R. China,Zhengzhou 450000
    3. Industrial Crops Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002
  • Received:2021-07-28 Online:2022-04-26 Published:2022-05-06
  • Contact: SU Xiu-hong E-mail:1739080605@qq.com;suxiuhong80@163.com

Abstract:

The development of dominant strain resources from Rehmannia glutinosa and the analysis of growth promoting characteristics will provide a basis for the development of special growth-promoting bacterial fertilizer for R. glutinosa microorganisms. Dilution plate method was used to classify the endophytic bacteria isolated from the roots,stems and leaves of R. glutinosa at different stages to their morphologies,physiologies and biochemistries and 16S rRNA analysis. And the selected 54 strains of endophytic bacteria were evaluated based on their IAA-yielding capacities,iron-carrier activities,P-dissolving capacities,and N-fixing capacities. The results showed that 512 strains of endophytic bacteria were isolated in different periods and classified into 54 groups. One strain in each group was selected for detection,of which 41 strains had the ability to produce IAA;29 strains of them had the ability of dissolving phosphorus;28 strains had the ability to produce iron carrier;and 25 strains had nitrogen fixation ability. This result provides a reference for the study of special microbial growth-promoting bacteria fertilizer for R. glutinosa,and lays a foundation for the subsequent application of microbial growth-promoting bacteria fertilizer to the field experiment of R. glutinosa.

Key words: Rehmannia glutinosa., isolation and identification, endophytic bacteria, growth-promoting