Identification of AHP Gene Family in Cucumis sativus and Its Expression Analysis Under Abiotic Stress

doi:10.13560/j.cnki.biotech.bull.1985.2021-1338

Abstract

Abstract:

Histidine-phosphate transporter（AHP）is one of the important proteins in cytokinin signal transduction pathway,which plays an important role in plant growth and development and resistance to a series of abiotic stresses. Exploring the molecular characteristics and expression pattern of AHP in C. sativus can provide theoretical basis for further exploring the function of AHP gene in C. sativus and improving abiotic stress tolerance of plants. The number,evolutionary relationship,cis-acting elements,gene structure,development and expression patterns of AHP gene family members under abiotic stress in C. sativus were analyzed. The results showed that there were a total of 7 members of AHP gene family in C. sativus,which were unevenly distributed on 6 chromosomes. Phylogenetic analysis indicated that the AHP gene family members of C. sativus were closely related to Benincasa hispida and Citrullus vulgaris Schrad L. Its promoter sequence mainly contained cis-acting elements including light response,low temperature response,drought response,hormone response and defense stress. Transcriptome analysis showed that CsAHP1,CsAHP3 and CsAHP4 were significantly up-regulated or down-regulated under abiotic stress and the expressions of CsAHP1 was the highest on flowering day. Furthermore,real-time fluorescence quantitative PCR（qRT-PCR）results indicated that CsAHP1 and CsAHP3 were significantly up-regulated under drought and high temperature stress,and CsAHP1,CsAHP2 and CsAHP5 were significantly up-regulated under low temperature and low light stress. The AHP gene family of C. sativus might be closely related to plant development and abiotic stress response.

Key words: C. sativus L., histidine phosphate transporter, abiotic stress, gene expression

ZHOU Guo-yan, YIN Shan-shan, GAO Jia-xin, WU Chun-cheng, YAN Li-ying, XIE Yang. Identification of AHP Gene Family in Cucumis sativus and Its Expression Analysis Under Abiotic Stress[J]. Biotechnology Bulletin, 2022, 38(6): 112-119.

Figures/Tables 7

Table 1 qRT-PCR primer design

引物名称Primer name	引物序列Primer sequence（5'-3'）
CsAHP1-F	TTGTTGAAGTGGTGTCTC
CsAHP1-R	CCTATGCTTGAACTGCTT
CsAHP2-F	TACTACAGAGACTCATCCA
CsAHP2-R	GGCATCCAACTTATTGAAG
CsAHP3-F	CCAATTCCTTCGCTACAA
CsAHP3-R	ATCAATACACTTCTGCTCAA
CsAHP4-F	AAGACAGTGGATTCAGTAT
CsAHP4-R	TAGCGATTCTCAGTTTATTG
CsAHP5-F	TTTGAGCAGGGTTATCTTG
CsAHP5-R	GTATGAGTCTGGCTGAATC
CsAHP6-F	GAACCGATTACTTGCCTTA
CsAHP6-R	TCATCTTGGAGTTGTTGTAG
CsAHP7-F	ATTGTTGAGGCAGATGAA
CsAHP7-R	CTATATGAATCACGGTAGCA
Cs-actin-F	ATTGTTCTCAGTGGTGGTTCTAC
Cs-actin-R	CCTTTGAGATCCACATCTGCT

Fig. 1 Chromosome location distribution map of AHP gene family members in C. sativus

Fig. 2 Phylogenetic tree analysis of AHP family members in C. sativus,Arabidopsis thaliana and Cucurbitaceae Cs：黄瓜Cucumis sativus L.;At：拟南芥Arabidopsis thaliana L.;Cmo：南瓜Cucurbita moschata Duch L.;Cla：西瓜Citrullus vulgaris Schrad L.;MEL：甜瓜Cucumis melo L.;Bhi：冬瓜Benincasa hispida L.;Cp：西葫芦Cucurbita pepo L.;Lsi：葫芦 Lagenaria siceraria L.

Fig. 3 Analysis of cis-acting element in promoter sequence of AHP family gene in C. sativus

Fig. 4 Gene structure and conservative domain analysis of AHP family in C. sativus

Fig. 5 Expression pattern analysis of AHP family in abiotic stress and different developmental stages of C. sativus a：Salt stress. b：Silicon stress. c：Temperature and light stress. d：Expression patterns in different organizations. e：Expression patterns of genes during anthesis

Fig. 6 qRT-PCR analysis of AHP family gene under drou-ght,high temperature,and low temperature and low light stress in C. sativus

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